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Journal of microbiological methods

CRISPR/dCas9-mediated inhibition of gene expression in Staphylococcus aureus.


PMID 28522389

Abstract

The understanding of the genetic mechanism of Staphylococcus aureus requires efficient tools, however, genetic manipulation in S. aureus is always laborious and time-consuming. Here we proposed a novel CRISPR/dCas9 interference method for the rapid knockdown of target genes. Furthermore, multiple genes can be repressed simultaneously by using this method.