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Biochemistry

Interactions of 4-nitroquinoline 1-oxide with four deoxyribonucleotides.


PMID 418803

Abstract

The interactions of 4-nitroquinoline 1-oxide (NQO) with the four 5'-deoxyribonucleotides were probed using absorption spectra of the charge transfer bands and 1H and 13C nuclear magnetic resonance (NMR) spectra of nucleotide-NQO mixtures. Spectral data yielded equilibrium constants (K(dpG:NQO) = 16 M-1, K(dpA:NQO) = 12 M-1, K(dpT:NQO) = K(dpC:NQO) = 4 M-1) which suggest the preference of NQO for the guanine residue in a DNA. This is in agreement with the data of Okano, T., et al. [(1969) Gann 60, 295]. From 13C and 1H NMR data on nucleosides, a structure for the dpG:NQO complex is proposed.