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Clinical chemistry

Automated kinetic determination of angiotensin-converting enzyme in serum.


PMID 6327119

Abstract

In this automated kinetic modification of a previous method (Anal Biochem 95: 540-548, 1979) for determining angiotensin-converting enzyme (EC 3.4.15.1), 3-(2- furylacryloyl )-L- phenylalanylglycylglycine is used as the substrate. The change in absorbance at 340 nm is used to monitor hydrolysis of the substrate. The rate of hydrolysis is roughly threefold greater than with previously reported substrates, so assay time and sensitivity are improved.

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F7131
N-[3-(2-Furyl)acryloyl]-Phe-Gly-Gly
C20H21N3O6