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Biokhimiia (Moscow, Russia)

[Protein determination by binding with the dye Coomassie brilliant blue G-250].


PMID 7521220

Abstract

Possible applications of the Coomassie brilliant blue G-250 dye method for protein assay are reviewed. The physico-chemical and optical properties of the dye are considered in detail. The data on the protein-binding mechanism are generalized. Modifications of the Coomassie blue G-250 quantitative protein assays are critically considered. The applications of the method in medicine, biochemistry and biotechnology are discussed.