Agarose Type IX-A, Ultra-low Gelling Temperature

Properties

Related Categories	Agarose, Core Bioreagents, Life Science Reagents for DNA/RNA Electrophoresis, Life Science Reagents for Northern and Southern Blotting, Life Science Reagents for Nucleic Acid Purification, Life Science Reagents for PCR, Life Science Reagents for Transfection, Molecular Biology, Nucleic Acid Electrophoresis, Research Essentials More...
type	Type IX-A
impurities	≤7% water
ash	≤0.4%
EEO	≤0.12
transition temp	gel point ≤20 °C
gel strength	≥300 g/cm² (1.5% gel)
anion traces	sulfate (SO₄^2-): ≤0.14%

Description

Analysis Note

The following is a list of properties associated with our agaroses:
Sulfate content - used as an indicator of purity, since sulfate is the major ionic group present.
Gel strength - the force that must be applied to a gel to cause it to fracture.
Gel point - the temperature at which an aqueous agarose solution forms a gel as it cools. Agarose solutions exhibit hysteresis in the liquid-to-gel transition - that is, their gel point is not the same as their melting temperature.
Electroendosmosis (EEO) - a movement of liquid through the gel. Anionic groups in an agarose gel are affixed to the matrix and cannot move, but dissociable counter cations can migrate toward the cathode in the matrix, giving rise to EEO. Since electrophoretic movement of biopolymers is usually toward the anode, EEO can disrupt separations because of internal convection.

Application

Yields unusually strong gels for an ultra-low gelling agarose. Ideal for electrophoresis of heat-labile samples and for growth of hybridomas or other cell lines.