Synonym: Acid Blue 83, Brilliant Blue R, Brilliant indocyanin 6B, Coomassie Brilliant Blue R
|Related Categories||B, Cell Biology, Coomassie Stains, Core Bioreagents, Hematology and Histology,|
|suitability||suitable for SDS-PAGE|
Brilliant Blue R concentrate is a methanol based stain especially designed for staining protein in polyacrylamide but will also stain agarose gels. The stain contains methanol and acetic acid so gels do not require fixing prior to staining.
For detection of protein bands following electrophoresis.
After dilution with deionized water to a final volume of one liter, solution will contain 0.25% (w/v) Brilliant Blue R, 40% (v/v) methanol and 7% (v/v) acetic acid.
Certificate of Analysis
Certificate of Origin
|Symbol||GHS02, GHS06, GHS08|
|Hazard statements||H225-H301 + H311 + H331-H315-H319-H370|
|Precautionary statements||P210-P260-P280-P301 + P310-P305 + P351 + P338-P311|
|Personal Protective Equipment||Faceshields, full-face respirator (US), Gloves, Goggles, multi-purpose combination respirator cartridge (US), type ABEK (EN14387) respirator filter|
|Hazard Codes (Europe)||F,T|
|Risk Statements (Europe)||11-23/24/25-36/38-39/23/24/25|
|Safety Statements (Europe)||16-26-36/37-45|
|RIDADR||UN 1230 3/PG 2|
|Flash Point(F)||57.2 °F|
|Flash Point(C)||14 °C|
Preparation of Mg-Al layered double hydroxide intercalated with Coomassie Brilliant Blue R anion and its uptake of aromatic compounds from aqueous solutions. Kameda T, Sato S, and Yoshioka T J. Environ. Sci. Health. A. Tox. Hazard. Subst. Environ. Eng. 47(13), 2035-9, (2012)
Enhanced stability and decolorization of Coomassie Brilliant Blue R-250 by dextran aldehyde-modified horseradish peroxidase. Altikatoglu M and Celebi M Artif. Cells Blood Substit. Immobil. Biotechnol. 39(3), 185-90, (2011)
Sodium dodecyl sulphate-polyacrylamide gel electrophoresis of human parotid salivary proteins: comparison of dansylation, coomassie blue R-250 and silver detection methods. Beeley JA, Newman F, Wilson PH, et al. Electrophoresis 17, 505-506, (1996)
A modified coomassie brilliant blue G 250 staining method for the detection of chitinase activity and molecular weight after polyacrylamide gel electrophoresis. Liau CY and Lin CS J. Biosci. Bioeng. 106(1), 111-3, (2008)
High MS-compatibility of silver nitrate-stained protein spots from 2-DE gels using ZipPlates and AnchorChips for successful protein identification. Nebrich G, Herrmann M, Sagi D, et al. Electrophoresis 28(10), 1607-14, (2007)
Mass spectrometric characterization of low-molecular-mass color pI markers and their use for direct determination of pI value of proteins. Mazanec K, Slais K, and Chmelík J J. Mass Spectrom. 41(12), 1570-7, (2006)
Peptide mass fingerprint sequence coverage from differently stained proteins on two-dimensional electrophoresis patterns by matrix assisted laser desorption/ionization-mass spectrometry (MALDI-MS). Scheler C, Lamer S, Pan Z, et al. Electrophoresis 19(6), 918-27, (1998)
Aldrich MSDS 1, 222:C / Corp MSDS 1 (1), 489:B / FT-IR 1 (2), 1040:B / FT-IR 2 (3), 4133:C / IR-Spectra (3), 1487:G / RegBook 1 (2), 2779:D / Sigma FT-IR 1 (2), 107:C / Stains and Dyes Ref, 153 / Structure Index 1, 441:C:8
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