Nitro Blue Tetrazolium tablet

Properties

Related Categories	Alkaline Phosphatase, Alphabetical Listing, Biochemicals and Reagents, Colorimetric, Core Bioreagents, Detection, Detection Substrates, Enzyme Substrates, Enzymes, Inhibitors, and Substrates, Life Science Reagents for Western Blotting, Research Essentials, Stains and Dyes, Substrates, Tablets, Western Blotting More...
form	tablet
storage temp.	2-8°C

Description

Quantity

Contains 10 mg substrate per tablet.

Specificity

Nitro Blue Tetrazolium is used in conjunction with the alkaline phosphatase substrate 5-Bromo-4-Chloro-3-Indolyl Phosphate (BCIP) Tablets (Sigma Product No. B0274) in immunoblotting and immunohistological staining procedures. This substrate system produces an insoluble end product that is blue-purple in color and can be observed visually.

Price and Availability

Related Papers

A rapid, sensitive method for detection of alkaline phosphatase-conjugated anti-antibody on Western blots. Blake, M.S. Anal. Biochem. 136, 175, (1984)

Documents

Certificate of Analysis

N5514 - Datasheet (46 KB)

Safety Information

Symbol	GHS07
Signal word	Warning
Hazard statements	H319
Precautionary statements	P305 + P351 + P338
Personal Protective Equipment	dust mask type N95 (US), Eyeshields, Gloves
Hazard Codes (Europe)	Xn
Risk Statements (Europe)	22-36
Safety Statements (Europe)	26
WGK Germany	3

Technical information & documentation associated with this product is available in the Safety & Documentation tab.

Articles

Colorimetric Alkaline Phosphatase and Peroxidase Substrate Detection Systems

Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrat...
BioFiles 2008, 3.4, 6.
Keywords: Enzyme-linked immunosorbent assay, Immunohistochemistry, Western blot