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P7949 Sigma-Aldrich

TWEEN® 20

BioXtra, viscous liquid

Synonym: Polyethylene glycol sorbitan monolaurate, Polyoxyethylenesorbitan monolaurate

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Properties

Related Categories Antigen-Vaccine Preparation, Biochemicals and Reagents, Cell Culture, Core Bioreagents, Detergents,
description   non-ionic
product line   BioXtra
form   viscous liquid
mol wt   mol wt ~1228
impurities   ≤0.05% Phosphorus (P)
ign. residue   ≤0.2%
refractive index   n20/D 1.468(lit.)
CMC   0.06 mM(20-25°C)
transition temp   cloud point 76 °C
density   1.095 g/mL at 25 °C(lit.)
anion traces   chloride (Cl-): ≤0.05%
  sulfate (SO42-): ≤0.05%
cation traces   Al: ≤0.0005%
  Ca: ≤0.0005%
  Cu: ≤0.0005%
  Fe: ≤0.0005%
  K: ≤0.005%
  Mg: ≤0.0005%
  NH4+: ≤0.05%
  Na: ≤0.05%
  Pb: ≤0.001%
  Zn: ≤0.0005%
HLB   16.7

Description

Frequently Asked Questions

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Application

Non-ionic detergent

TWEEN 20 is a nonionic detergent widely used in biochemical applications. It has been used as an emulsifying agent for the preparation of stable oil-in-water emulsions. TWEEN 20 has been used in pre-extraction of membranes to remove peripheral proteins (used at 2% for extraction of membrane-bound proteins).
TWEEN 20 has been used as a blocking agent for membrane based immunoassays at a typical concentration of 0.05%. TWEEN 20 can be used for lysing mammalian cells at a concentration of 0.005 to 0.5%.

Caution

TWEEN 20 may not be stable to autoclaving, particularly with metal cations in buffer solutions. Autoclaving is not recommended without testing for changes in properties. TWEEN 20 is heat sensitive and will darken when exposed to elevated temperatures. Polysorbates have been reported to be incompatible with alkalis, heavy metal salts, phenols, and tannic acid. They may also reduce the activity of many preservatives. Aqueous solutions of polysorbates undergo autoxidation during storage, with changes being catalyzed by light, increased temperature, and copper sulfate.

Preparation Note

TWEEN 20 is miscible in water (100mg/mL), yielding a clear, yellow solution. It is also miscible with alcohol, dioxane, and ethyl acetate. TWEEN 20 is insoluble in liquid paraffin and fixed oils.

Legal Information

TWEEN is a registered trademark of Croda International PLC

Price and Availability

Suggested Laboratory Gloves


Laboratory GlovesThis substance has been tested against several types of hand protection for CE compliance. Click below to find the recommended gloves for handling this product.



TruPAGE™ Pre-cast Polyacrylamide Gels


TruPAGE™ Pre-cast Polyacrylamide GelsTruPAGE Gels run true every time!

  • . 2-Year Shelf Life
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for electrophoresis, suitable for solubilizing agent of membrane proteins and as a blocking reagent in immunoblotting

Safety & Documentation

Safety Information

Personal Protective Equipment 
WGK Germany 
1
RTECS 
TR7400000
Flash Point(F) 
>230 °F
Flash Point(C) 
>110 °C

Protocols & Articles

Peer-Reviewed Papers

References

Set your institution to view full text papers.

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Enhanced expressions of microvascular smooth muscle receptors after focal cerebral ischemia occur via the MAPK MEK/ERK pathway. Maddahi A BMC Neurosci. 9, 85, (2008)

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Sphingosine kinases and sphingosine-1-phosphate are critical for transforming growth factor beta-induced extracellular signal-regulated kinase 1 and 2 activation and promotion of migration and invasion of esophageal cancer cells. Miller AV Mol. Cell. Biol. 28(12), 4142-51, (2008)

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Membrane domain specificity in the spatial distribution of aquaporins 5, 7, 9, and 11 in efferent ducts and epididymis of rats. Hermo, L J. Histochem. Cytochem. 56(12), 1121-35, (2008)

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Role of ERK/MAPK in endothelin receptor signaling in human aortic smooth muscle cells. Chen QW BMC Cell Biol. 10, 52, (2009)

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Dysbindin-1 in dorsolateral prefrontal cortex of schizophrenia cases is reduced in an isoform-specific manner unrelated to dysbindin-1 mRNA expression. Tang J Hum. Mol. Genet. 18(20), 3851-63, (2009)

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Formaldehyde-based whole-mount in situ hybridization method for planarians. Bret J. Pearson et al Dev. Dyn. 238, 443-50, (2009)

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Evaluation of analytical and preliminary clinical performance of Myconostica MycAssay Aspergillus when testing serum specimens for diagnosis of invasive Aspergillosis. P. Lewis White et al J. Clin. Microbiol. 49, 2169-74, (2011)

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Retinoid metabolism and ALDH1A2 (RALDH2) expression are altered in the transgenic adenocarcinoma mouse prostate model. Touma SE Biochem. Pharmacol. 78(9), 1127-38, (2009)

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Intra- and inter-clade cross-reactivity by HIV-1 Gag specific T-cells reveals exclusive and commonly targeted regions: implications for current vaccine trials. Zembe L, Burgers WA, Jaspan HB, et al. PLoS ONE 6(10), e26096, (2011)

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Fine antigenic specificity and cooperative bactericidal activity of monoclonal antibodies directed at the meningococcal vaccine candidate factor h-binding protein. Beernink PT Infect. Immun. 76(9), 4232-40, (2008)

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The Drosophila fragile X mental retardation gene regulates sleep need. Bushey D J. Neurosci. 29(7), 1948-61, (2009)

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The rapid decrease in astrocyte-associated dystroglycan expression by focal cerebral ischemia is protease-dependent. Milner R J. Cereb. Blood Flow Metab. 28(4), 812-23, (2008)

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Increased expression of glutathione peroxidase 4 strongly protects retina from oxidative damage. Lu L Antioxid. Redox Signal. 11(4), 715-24, (2009)

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Screening for resistance against Pseudomonas syringae in rice-FOX Arabidopsis lines identified a putative receptor-like cytoplasmic kinase gene that confers resistance to major bacterial and fungal pathogens in Arabidopsis and rice. Dubouzet JG Plant Biotechnol. J. 9(4), 466-85, (2011)

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Direct Repeat 6 from human herpesvirus-6B encodes a nuclear protein that forms a complex with the viral DNA processivity factor p41. Schleimann MH PLoS ONE 4(10), e7457, (2009)

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Retinoic acid-treated pluripotent stem cells undergoing neurogenesis present increased aneuploidy and micronuclei formation. Sartore, RC PLoS ONE 6(6), e20667, (2011)

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Influenza H5 hemagglutinin DNA primes the antibody response elicited by the live attenuated influenza A/Vietnam/1203/2004 vaccine in ferrets. Suguitan AL PLoS ONE 6(7), e21942, (2011)

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Beneficial effects of a novel RAGE inhibitor on early diabetic retinopathy and tactile allodynia. Guangyuan Li et al Mol. Vis. 17, 3156-65, (2011)

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Modulation of astrocyte P2Y1 receptors by the carboxyl terminal domain of the gap junction protein Cx43. Scemes E Glia 56(2), 145-53, (2008)

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Increased operant responding for ethanol in male C57BL/6J mice: specific regulation by the ERK1/2, but not JNK, MAP kinase pathway. Faccidomo S Psychopharmacology 204(1), 135-47, (2009)

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Interferon-λ1 (interleukin-29) preferentially down-regulates interleukin-13 over other T helper type 2 cytokine responses in vitro. Shekar Srinivas et al Immunology 125, 492-502, (2008)

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A mitotic GlcNAcylation/phosphorylation signaling complex alters the posttranslational state of the cytoskeletal protein vimentin. Slawson C Mol. Biol. Cell 19(10), 4130-40, (2008)

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Multiple functional domains of Enterococcus faecalis aggregation substance Asc10 contribute to endocarditis virulence. Olivia N. Chuang et al Infect. Immun. 77, 539-48, (2009)

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Arabidopsis seedling flood-inoculation technique: a rapid and reliable assay for studying plant-bacterial interactions. Ishiga Y Plant Methods 7, 32, (2011)

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Gla-rich protein is a novel vitamin K-dependent protein present in serum that accumulates at sites of pathological calcifications. Viegas CS Am. J. Pathol. 175(6), 2288-98, (2009)

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Inhibition of vascular endothelial growth factor receptor signal transduction blocks follicle progression but does not necessarily disrupt vascular development in perinatal rat ovaries. McFee RM Biol. Reprod. 81(5), 966-77, (2009)

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Design and analysis of rhesus cytomegalovirus IL-10 mutants as a model for novel vaccines against human cytomegalovirus. Naomi J. Logsdon et al PLoS ONE 6, e28127, (2011)

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Identification of human intracellular targets of the medicinal Herb St. John's Wort by chemical-genetic profiling in yeast. McCue PP J. Agric. Food Chem. 56(22), 11011-7, (2008)

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Combining milatuzumab with bortezomib, doxorubicin, or dexamethasone improves responses in multiple myeloma cell lines. Stein R, Smith MR, Chen S, et al. Clin. Cancer Res. 15(8), 2808-17, (2009)

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Reconstitution of Schwannian stroma in neuroblastomas using human bone marrow stromal cells. Wenlin Du et al Am. J. Pathol. 173, 1153-64, (2008)

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Serine residue 115 of MAPK-activated protein kinase MK5 is crucial for its PKA-regulated nuclear export and biological function. Kostenko, S., et al. Cell. Mol. Life Sci. 68, 847-62, (2011)

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DIDS protects against neuronal injury by blocking Toll-like receptor 2 activated-mechanisms. Hang Yao et al J. Neurochem. 108, 835-46, (2009)

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The heme precursor delta-aminolevulinate blocks peripheral myelin formation. Felitsyn N J. Neurochem. 106(5), 2068-79, (2008)

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Diagnosis of prostate cancer by detection of minichromosome maintenance 5 protein in urine sediments. Dudderidge TJ Br. J. Cancer 103(5), 701-7, (2010)

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Transformation of MCF-10A cells by random mutagenesis with frameshift mutagen ICR191: a model for identifying candidate breast-tumor suppressors. Zientek-Targosz H Mol. Cancer 7, 51, (2008)

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Loss of runt-related transcription factor 3 expression leads hepatocellular carcinoma cells to escape apoptosis. Nakanishi Y BMC Cancer 11, 3, (2011)

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The cyclooxygenase inhibitor sulindac sulfide inhibits EP4 expression and suppresses the growth of glioblastoma cells. Kambe A, Yoshioka H, Kamitani H, et al. Cancer Prev. Res. (Phila.) 2(12), 1088-99, (2009)

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Effect of MOG sensitization on somatosensory evoked potential in Lewis rats. All AH J. Neurol. Sci. 284(1-2), 81-9, (2009)

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Genetic dissection of gamma-secretase-dependent and -independent functions of presenilin in regulating neuronal cell cycle and cell death. Kallhoff-Munoz V J. Neurosci. 28(44), 11421-31, (2008)

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Secreted osteopontin is highly polymerized in human airways and fragmented in asthmatic airway secretions. Arjomandi, M PLoS ONE 6(10), e25678, (2011)

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Characterization of trans-neuronal trafficking of Cbln1. Wei P Mol. Cell. Neurosci. 41(2), 258-73, (2009)

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Protease-activated receptor 2 is involved in Th2 responses against Trichinella spiralis infection. Mi Kyung Park et al Korean J. Parasitol. 49, 235-43, (2011)

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Identification of Mycobacterium tuberculosis ornithine carboamyltransferase in urine as a possible molecular marker of active pulmonary tuberculosis. Napolitano DR Clin. Vaccine Immunol. 15(4), 638-43, (2008)

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Expression of RHOGTPase regulators in human myometrium. O'Brien M Reprod. Biol. Endocrinol. 6, 1, (2008)

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Identification of amino acid residues responsible for von Willebrand factor binding to sulfatide by charged-to-alanine-scanning mutagenesis. Nakayama T Int. J. Hematol. 87(4), 363-70, (2008)

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Muscle differentiation in a colonial ascidian: organisation, gene expression and evolutionary considerations. Degasperi V BMC Dev. Biol. 9, 48, (2009)

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Molecular and immunocytochemical characterization of primary neuronal cultures from adult rat brain: Differential expression of neuronal and glial protein markers. Balmiki Ray et al J. Neurosci. Methods 184, 294-302, (2010)

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High-throughput fluorescence-based screening assays for tryptophan-catabolizing enzymes. Seegers N, van Doornmalen AM, Uitdehaag JC, et al. J. Biomol. Screen. 19(9), 1266-74, (2014)

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Point mutation in the mouse P2X7 receptor affects intercellular calcium waves in astrocytes. Suadicani SO ASN Neuro 1(1), doi:10.1042/AN20090001, (2009)

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A novel biologic immunomodulator, HDFx, protects against lethal hemorrhage, endotoxins and traumatic injury: potential relevance to emerging diseases. Altura BM Int. J. Clin. Exp. Med. 2(3), 266-79, (2009)

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An estrogen receptor α activity indicator model in mice. Sang Jun Han et al Genesis 47, 815-24, (2009)

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In vivo and in vitro techniques for comparative study of antiviral T-cell responses in the amphibian Xenopus. Morales, H Biol. Proced. Online 10, 1-8, (2008)

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Fenaroli / RegBook 1 (1), 761:E / Sax 6, 2260

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