EMAIL THIS PAGE TO A FRIEND

P7949 Sigma-Aldrich

TWEEN® 20

BioXtra, viscous liquid

Synonym: Polyethylene glycol sorbitan monolaurate, Polyoxyethylenesorbitan monolaurate

Properties

Related Categories Antigen-Vaccine Preparation, Biochemicals and Reagents, Cell Culture, Core Bioreagents, Detergents,
description   non-ionic
product line   BioXtra
form   viscous liquid
mol wt   mol wt ~1228
impurities   ≤0.05% Phosphorus (P)
ign. residue   ≤0.2%
refractive index   n20/D 1.468(lit.)
CMC   0.06 mM(20-25°C)
transition temp   cloud point 76 °C
density   1.095 g/mL at 25 °C(lit.)
anion traces   chloride (Cl-): ≤0.05%
  sulfate (SO42-): ≤0.05%
cation traces   Al: ≤0.0005%
  Ca: ≤0.0005%
  Cu: ≤0.0005%
  Fe: ≤0.0005%
  K: ≤0.005%
  Mg: ≤0.0005%
  NH4+: ≤0.05%
  Na: ≤0.05%
  Pb: ≤0.001%
  Zn: ≤0.0005%
HLB   16.7

Description

Frequently Asked Questions

Live Chat and Frequently Asked Questions are available for this Product.

Application

Non-ionic detergent

TWEEN 20 is a nonionic detergent widely used in biochemical applications. It has been used as an emulsifying agent for the preparation of stable oil-in-water emulsions. TWEEN 20 has been used in pre-extraction of membranes to remove peripheral proteins (used at 2% for extraction of membrane-bound proteins).
TWEEN 20 has been used as a blocking agent for membrane based immunoassays at a typical concentration of 0.05%. TWEEN 20 can be used for lysing mammalian cells at a concentration of 0.005 to 0.5%.

Caution

TWEEN 20 may not be stable to autoclaving, particularly with metal cations in buffer solutions. Autoclaving is not recommended without testing for changes in properties. TWEEN 20 is heat sensitive and will darken when exposed to elevated temperatures. Polysorbates have been reported to be incompatible with alkalis, heavy metal salts, phenols, and tannic acid. They may also reduce the activity of many preservatives. Aqueous solutions of polysorbates undergo autoxidation during storage, with changes being catalyzed by light, increased temperature, and copper sulfate.

Preparation Note

TWEEN 20 is miscible in water (100mg/mL), yielding a clear, yellow solution. It is also miscible with alcohol, dioxane, and ethyl acetate. TWEEN 20 is insoluble in liquid paraffin and fixed oils.

Legal Information

TWEEN is a registered trademark of Croda International PLC

Price and Availability


New TruPAGE™ Pre-cast Polyacrylamide Gels

Customers Also Viewed

TWEEN<SUP>®</SUP> 20

viscosity 250-450 mPa.s (25 °C)

TWEEN<SUP>®</SUP> 20

viscous liquid

TWEEN<SUP>®</SUP> 20

for molecular biology, viscous liquid

TWEEN<SUP>®</SUP> 20

viscous liquid, cell culture tested

TWEEN<SUP>®</SUP> 20

for electrophoresis, suitable for solubilizing agent of membrane proteins and as a blocking reagent in immunoblotting

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?