Dihydrofolate Reductase from bovine liver ~8 U/mL, ammonium sulfate suspension, off-white

Properties

Related Categories	1.5.x.x Acting on CH-NH, 1.x.x.x Oxidoreductases, Biochemicals and Reagents, Enzyme Class Index, Enzymes, Inhibitors, and Substrates, General Metabolic Enzymes, General Metabolic Enzymes A-H, Metabolomics More...
form	ammonium sulfate suspension
activity	≥6 U/mg protein
concentration	~8 U/mL
color	off-white
density	1.2 g/mL at 20 °C
storage temp.	2-8°C

Description

Other Notes

In folate metabolism for the synthesis of purines, thymidine and glycine³

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Unit Definition

1 U corresponds to the amount of enzyme which converts 1 μmol 7,8-dihydrofolate and NADPH to 5,6,7,8-tetrahydrofolate and NADP per minute at pH 6.5 and 25 °C

Physical form

Suspension in 3.6 M ammonium sulfate solution, pH 7.0

Application

Dihydrofolate reductase (DHFR) is involved in the synthesis of purines, thymidine and glycine in folate metabolism. DHFR, from bovine liver, is used for methotrexate analysis ¹. DHFR is useful to study various cancers such as sarcomas, leukemias and head and neck cancers ².

Biochem/physiol Actions

Dihydrofolate reductase (DHFR) reduces dihydrofolic acid to tetrahydrofolic acid, using NADPH as an electron donor. The binding of antitumor agents, such as methotrexate, to DHFR prevents the formation of reduced folates, which are essential for DNA synthesis ².