|Related Categories||A-C, Cell Culture, ECACC Cell Lines More...|
|biological source||kidney from hamster|
|shipped in||dry ice|
Transformation and virus studies
For complete product information, please see BHK TK-
DMEM or GMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:4 to 1:10 i.e. seeding at 1-2x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C.
BHK TK- is a derivative of BHK21 (clone 13) rendered TK- by selection in 30μg/ml BUdR. Used widely for research on viruses and on neoplastic transformation especially with polyoma virus.
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Glasgow Minimum Essential Medium, With sodium bicarbonate, without
Dulbecco’s Modified Eagle’s Medium - high glucose, With 4500 mg/L glucose, sodium pyruvate, and sodium bicarbonate, without
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