Properties
| Related Categories | A-C, Cell Culture, ECACC Cell Lines More... |
| biological source | ovary from hamster |
| growth mode | Adherent |
| karyotype | 2n = 22 |
| morphology | Epithelial |
| shipped in | dry ice |
| storage temp. | −196°C |
Description
Application
Genetic studies
General description
For complete product information, please see A2H
Culture Medium
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:2 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C.
Cell Line Description
Used for the induction and isolation of nutritionally deficient mutants. A reclone of ′Clone A′ established by Puck (the ′H′ denotes MRC Harwell). Useful for genetic studies due to the low chromosome number.
Price and Availability
NEW! Sigma® Cultureware
Introducing Sigma’s new competitively priced line of cultureware:
- . Multiwell Plates
- . Culture Flasks
- . Cryogenic Vials & More
Technical Service:
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Bulk Ordering & Pricing:
Need larger quantities for your development, manufacturing or research applications?
