|Related Categories||A-C, Cell Culture, ECACC Cell Lines More...|
|biological source||nerve from rat|
|shipped in||dry ice|
For complete product information, please see B92
DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:3 i.e. seeding at 4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C.
Ethyl nitrosaurea induced tumour cell line. Neuronal and glial cells are useful for research into the distribution of neurotransmitter synthesis and brain specific antigens among nerve and glia. Morphology significantly affected when grown in serum free media.
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Dulbecco’s Modified Eagle’s Medium - high glucose, With 4500 mg/L glucose, sodium pyruvate, and sodium bicarbonate, without
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