Properties
| Related Categories | A-C, Cell Culture, ECACC Cell Lines More... |
| biological source | Kidney from feline |
| growth mode | Adherent |
| karyotype | Hypodiploid, modal no 36 |
| morphology | Epithelial |
| products | Carcinoembryonic antigen (CEA) |
| shipped in | dry ice |
| storage temp. | −196°C |
Description
Application
Virus studies
General description
For complete product information, please see CRFK (BVD Ag negative)
Culture Medium
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:3 to 1:8 i.e. seeding at 1-3x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C.
Cell Line Description
CRFK cells were isolated from the cortical portion of the kidneys of a 10-12 week old normal female domestic cat. CRFK are extensively used in feline virus research. Susceptible to herpesvirus and feline picornaviruses producing CPE. BVD Ag negative.
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