|Related Categories||A-C, Cell Culture, ECACC Cell Lines More...|
|biological source||Kidney from feline|
|karyotype||Hypodiploid, modal no 36|
|products||Carcinoembryonic antigen (CEA)|
|shipped in||dry ice|
For complete product information, please see CRFK (BVD Ag negative)
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:3 to 1:8 i.e. seeding at 1-3x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C.
CRFK cells were isolated from the cortical portion of the kidneys of a 10-12 week old normal female domestic cat. CRFK are extensively used in feline virus research. Susceptible to herpesvirus and feline picornaviruses producing CPE. BVD Ag negative.
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|M7145||MEM Non-essential Amino Acid Solution (100×), without L-glutamine, liquid, sterile-filtered, BioReagent, suitable for cell culture|
Minimum Essential Medium Eagle, With Earle′s salts and sodium bicarbonate, without
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