Properties
| Related Categories | A-C, Cell Culture, ECACC Cell Lines More... |
| biological source | Cervix from human |
| growth mode | Adherent |
| karyotype | 2n = 46 |
| morphology | Epithelial |
| shipped in | dry ice |
| storage temp. | −196°C |
Description
Application
Virus studies: poliovirus 1
General description
For complete product information, please see AV3 (HeLa derivative)
DNA Profile
STR-PCR Data: Amelogenin: X
CSF1PO: 9,10
D13S317: 14
D16S539: 9,10
D5S818: 11,12
D7S820: 12
THO1: 7
TPOX: 8,12
vWA: 16,18
Culture Medium
EMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 2-5x100,000 cells/cm2 using 0.25% trypsin; 5% CO2; 37°C.
Cell Line Description
This cell line was originally derived from normal human amnion. The cells are mainly used for virus studies (susceptible to poliovirus 1). HeLa marker chromosomes and type A G6PD have been detected. This cell line was found to be indistinguishable from HeLa by STR PCR DNA profiling. Therefore, the cell line must be considered to be derived from HeLa.
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