90011810 Sigma

161BR

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Properties

Related Categories 0-10, Cell Culture, ECACC Cell Lines More...
biological source   skin from human
growth mode   Adherent
morphology   Fibroblast
shipped in   dry ice
storage temp.   −196°C

Description

Application

DNA repair studies

General description

For complete product information, please see 161BR

DNA Profile

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 12,14
D16S539: 11
D5S818: 9,11
D7S820: 8,13
THO1: 6,9.3
TPOX: 8
vWA: 17,18

Culture Medium

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 15% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 3-5x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.

Cell Line Description

Established from a skin of a normal 49 year old male. The cells have been used in radiation survival studies.

Price and Availability

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