91072536 Sigma

J5

Reactivity: Anti-T Cell Activation Antigen, bovine

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Properties

Related Categories Cell Culture, ECACC Cell Lines, Hybridomas More...
biological source   from Mouse x Mouse Hybridoma
ImmunoDon   BALB/c mouse spleen
Myeloma   P3X63Ag8
isotype   IgG2b
shipped in   dry ice
storage temp.   −196°C

Description

Immunogen

Theileria parva infected T lymphoblast plasma membranes

General description

For complete product information, please see J5

Culture Medium

RPMI 1640 + 2mM glutamine + 10% Foetal Bovine Serum (FBS)

Subculture Routine

Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C. These cells may require mouse macrophage feeder cells upon resuscitation.

Cell Line Description

The antibody is directed against a 100kDa antigen present on T lymphocytes activated by ConA, PWM, alloantigens or Theileria parva transformation.

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