Properties
| Related Categories | A-C, Cell Culture, ECACC Cell Lines More... |
| biological source | brain from rat |
| growth mode | Adherent |
| products | Plasminogen activator activity |
| shipped in | dry ice |
| storage temp. | −196°C |
Description
Application
Study of carcinogenesis, tumourigenicity studies
General description
For complete product information, please see BE10-Late
Culture Medium
DMEM + 2mM Glutamine + 15% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:10 to 1:20 i.e. seeding at 1-2x100,000 cells/25cm2 using 0.25% trypsin/EDTA; 10-15% CO2; 37°C.
Cell Line Description
Malignant cells produced on passaging BE10-Intermediate (Sigma Catalogue number. 93040119) beyond passage 40. The cells possess tissue plasminogen activator activity, are tumourigenic in BDIX rats and form colonies in agar.
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