|Related Categories||A-C, Cell Culture, ECACC Cell Lines More...|
|biological source||brain from rat|
|products||Plasminogen activator activity|
|shipped in||dry ice|
Study of carcinogenesis, tumourigenicity studies
For complete product information, please see BE10-Late
DMEM + 2mM Glutamine + 15% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:10 to 1:20 i.e. seeding at 1-2x100,000 cells/25cm2 using 0.25% trypsin/EDTA; 10-15% CO2; 37°C.
Malignant cells produced on passaging BE10-Intermediate (Sigma Catalogue number. 93040119) beyond passage 40. The cells possess tissue plasminogen activator activity, are tumourigenic in BDIX rats and form colonies in agar.
Analysis of N-ethyl-N-nitrosourea-induced brain carcinogenesis by sequential culturing during the latent period. I. Morphology and tumorigenicity of the cultured cells and their growth in agar. Roscoe JP and Claisse PJ J. Natl. Cancer Inst. 61(2), 381-90, (1978)
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Dulbecco’s Modified Eagle’s Medium - high glucose, With 4500 mg/L glucose, sodium pyruvate, and sodium bicarbonate, without
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