|Related Categories||A-C, Cell Culture, ECACC Cell Lines More...|
|biological source||brain from rat|
|products||Plasminogen activator activity|
|shipped in||dry ice|
Study of carcinogenesis, tumourigenicity studies
For complete product information, please see BE10-Late
DMEM + 2mM Glutamine + 15% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:10 to 1:20 i.e. seeding at 1-2x100,000 cells/25cm2 using 0.25% trypsin/EDTA; 10-15% CO2; 37°C.
Malignant cells produced on passaging BE10-Intermediate (Sigma Catalogue number. 93040119) beyond passage 40. The cells possess tissue plasminogen activator activity, are tumourigenic in BDIX rats and form colonies in agar.
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Dulbecco’s Modified Eagle’s Medium - high glucose, With 4500 mg/L glucose, sodium pyruvate, and sodium bicarbonate, without
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