93052109 Sigma

KCB 89001

Properties

Related Categories Cell Culture, ECACC Cell Lines, J-N More...
biological source   Skin from deer
growth mode   Adherent
karyotype   2n = 62
morphology   Fibroblast-like
shipped in   dry ice
storage temp.   −196°C

Description

General description

For complete product information, please see KCB 89001

Culture Medium

Medium 199 + 2mM Glutamine + 15% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C.

Cell Line Description

Derived from the skin of an adult, female Sambar deer (Cervus unicolor), in January 1989.

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