|Related Categories||A-C, Cell Culture, ECACC Cell Lines More...|
|biological source||Lung (bronchus) from human|
|shipped in||dry ice|
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RPMI 1640 + 2mM Glutamine + 1mM Sodium Pyruvate (NaP) + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
This cell line was derived from a bronchogenic carcinoma of a 45-year-old male. The cells produce high levels of MUC-1 mucin mRNA, low levels of MUC-2 mRNA but do not express the MUC-3 gene.
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RPMI-1640 Medium, With sodium bicarbonate, without
|S8636||Sodium pyruvate solution, 100 mM, sterile-filtered, BioReagent, suitable for cell culture|
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