Properties
| Related Categories | A-C, Cell Culture, ECACC Cell Lines More... |
| biological source | Lung (bronchus) from human |
| growth mode | Adherent |
| morphology | Epithelial |
| shipped in | dry ice |
| storage temp. | −196°C |
Description
General description
For complete product information, please see ChaGo-K-1
Culture Medium
RPMI 1640 + 2mM Glutamine + 1mM Sodium Pyruvate (NaP) + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Cell Line Description
This cell line was derived from a bronchogenic carcinoma of a 45-year-old male. The cells produce high levels of MUC-1 mucin mRNA, low levels of MUC-2 mRNA but do not express the MUC-3 gene.
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