A2404 Sigma

Alcohol Oxidase solution from Pichia pastoris

buffered aqueous solution, 10-40 units/mg protein (biuret)

DOWNLOAD MSDS (PDF)

Synonym: Alcohol:oxygen oxidoreductase

Description

Unit Definition

One unit will oxidize 1.0 μmole of methanol to formaldehyde per min at pH 7.5 at 25 °C.

Physical form

Solution in phosphate-buffered 30% sucrose

Application

Alcohol Oxidase may be used to study protein translocation into peroxisomes. Product A2404 is from Pichia pastoris. It has been used for the bacterial expression and immunological verification of Hv-p68 cDNA clones 1.

Biochem/physiol Actions

Alcohol oxidase catalyzes the oxidation of short-chain, primary, aliphatic alcohols to their respective aldehydes. It has the highest affinity for methanol. Alcohol oxidase is involved in the metabolism of methanol, which is important for yeasts that utilize methanol as their sole energy source. Alcohol oxidase is primarily found in the peroxisome but may be present in the cytoplasm as well.

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