A3428 Sigma

Aprotinin from bovine lung

lyophilized powder, 3-8 TIU/mg solid, BioReagent, suitable for cell culture

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Synonym: Trasylol, Trypsin inhibitor (basic)

Description

Analysis Note

Another commonly used unit of activity is the KIU (Kallikrein Inhibitor Unit).
1 TIU ∼ 1,300 KIU.

General description

Note: When benzoyl-L-arginine ethyl ester (BAEE) is used as substrate one BAPNA unit is approx. 45 BAEE μmolar units at pH 8.0 at 25 °C or approx. 9,000 BAEE A253 units at pH 7.6 at 25 °C.

Aprotinin is a protein consisting of 58 amino acids, arranged in a single polypeptide chain that is crosslinked by three disulfide bridges.

Unit Definition

One trypsin inhibitor unit (TIU) will decrease the activity of 2 trypsin units by 50% where one trypsin unit will hydrolyze 1.0 μmole of Nα-benzoyl-DL-arginine p-nitroanilide (BAPNA) per min at pH 7.8 at 25°C.

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Aprotinin from bovine lung

lyophilized powder, 3-8 TIU/mg solid

Aprotinin from bovine lung

lyophilized powder, 3-7 TIU/mg solid

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