Myosin (Skeletal, Fast)?Alkaline Phosphatase monoclonal antibody | Immunofluorescence | A4335

Properties

Related Categories	Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies to Actin-associated Proteins/Myosin, Antibodies to Cytoskeleton, Clone Index, M, MUE-MZ, Primary Antibodies More...
species reactivity	feline, rat, bovine, chicken, human, mouse, rabbit, guinea pig
application(s)	direct immunofluorescence: 1:150 using formalin-fixed, paraffin-embedded human or animal skeletal muscle sections
clone	MY-32, monoclonal
antibody form	purified immunoglobulin
form	buffered aqueous glycerol solution
isotype	IgG1
shipped in	wet ice
storage temp.	2-8°C
Gene Information	human ... MYH1(4619), MYH2(4620) mouse ... Myh1(17879), Myh2(17882) rat ... Myh1(287408), Myh2(691644)

Description

Immunogen

rabbit muscle myosin.

Physical form

Solution in 0.05 M Tris buffer, pH 8.0, containing 1% bovine serum albumin, 1 mM MgCl₂, 50% glycerol, and 15 mM sodium azide.

Specificity

Localizes an epitope on the myosin heavy chain. Stains the fast (type II) and neonatal isomyosin molecules found in skeletal muscle, but does not stain cardiac muscle, smooth muscle or non-muscle myosin in cultured cells. Does react with human rhabdomyosarcomas.

Application

The level of mysosin (fast) in serum samples from sportsmen with past injury was determined by western blot using monoclonal mouse anti-myosin (skeletal/fast) as the primary antibody at a dilution of 1:90000.

Price and Availability

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