Synonym: Monoclonal Anti-Myosin (Skeletal, Fast) antibody produced in mouse
|Related Categories||Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies to Actin-associated Proteins/Myosin, Antibodies to Cytoskeleton,|
|species reactivity||feline, rat, bovine, chicken, human, mouse, rabbit, guinea pig|
|application(s)||direct immunofluorescence: 1:150 using formalin-fixed, paraffin-embedded human or animal skeletal muscle sections|
|antibody form||purified immunoglobulin|
|form||buffered aqueous glycerol solution|
|shipped in||wet ice|
human ... MYH1(4619), MYH2(4620)|
mouse ... Myh1(17879), Myh2(17882)
rat ... Myh1(287408), Myh2(691644)
rabbit muscle myosin.
Localizes an epitope on the myosin heavy chain. Stains the fast (type II) and neonatal isomyosin molecules found in skeletal muscle, but does not stain cardiac muscle, smooth muscle or non-muscle myosin in cultured cells. Does react with human rhabdomyosarcomas.
Solution in 0.05 M Tris buffer, pH 8.0, containing 1% bovine serum albumin, 1 mM MgCl2, 50% glycerol, and 15 mM sodium azide.
The level of mysosin (fast) in serum samples from sportsmen with past injury was determined by western blot using monoclonal mouse anti-myosin (skeletal/fast) as the primary antibody at a dilution of 1:90000.
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Keywords: Buffers, Cell biology, Epigenetics, Immunohistochemistry, Immunoprecipitation, Molecular biology, Neuroscience, Western blot
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