A7549 Sigma

Anti-Apoptosis-Inducing Factor antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

DOWNLOAD MSDS (PDF)

Synonym: Anti-AIF

Properties

Related Categories AO-AQ, Alphabetical Index, Antibodies, Antibodies for Apoptosis, Antibodies for Cell Biology,
species reactivity   human, mouse
application(s)   microarray: suitable
  western blot: 1:1,000 using human epitheloid carcinoma HeLa cell extract and mouse brain extract
clone   polyclonal
antibody form   IgG fraction of antiserum
form   buffered aqueous solution
mol wt   antigen mol wt 57 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... AIFM1(9131)
mouse ... Aifm1(26926)

Description

Immunogen

synthetic peptide corresponding to the C-terminus of human Apoptosis Inducing Factor (AIF), (amino acids 593-613), conjugated to KLH.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Application

Protein lysates from 2CLL, 10 MCl and Jeko-1 cells were analyzed by western blot using anti-AIF as the primary antibody. Cells also underwent intracellular delivery by being transfected with anti-AIF at a final dilution of 1:2500 and then analyzed by FLOW cytometry. 1

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