|Related Categories||Alkaline Phosphatase, Alphabetical Listing, Biochemicals and Reagents, Colorimetric, Detection,|
|usage||sufficient for 2 L working substrate|
Prior to reaction with alkaline phosphatase, the BCIP reagent is colorless to faint blue solution, and the Nitro BT reagent is a yellow solution. The two component mixture develops a bluish-purple product when reacted with alkaline phosphatase in microwell type assays. This substrate is not recommended for membrane or immunohistochemical type assays that require a precipitation reaction product.
A 2 component kit sufficient for preparing 2 liters of working substrate.
Supplied as a 2 component buffered alkaline phosphatase substrate containing Bromo-Chloro-Indolylphosp
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Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrat...
BioFiles 2008, 3.4, 6.
Keywords: Enzyme-linked immunosorbent assay, Immunohistochemistry, Western blot
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