AB0200 Sigma

Alkaline Phosphatase Blue Microwell Substrate

sufficient for 2 L working substrate

DOWNLOAD MSDS (PDF)

Description

Application

Prior to reaction with alkaline phosphatase, the BCIP reagent is colorless to faint blue solution, and the Nitro BT reagent is a yellow solution. The two component mixture develops a bluish-purple product when reacted with alkaline phosphatase in microwell type assays. This substrate is not recommended for membrane or immunohistochemical type assays that require a precipitation reaction product.

Physical form

A 2 component kit sufficient for preparing 2 liters of working substrate.

Supplied as a 2 component buffered alkaline phosphatase substrate containing Bromo-Chloro-Indolylphosphate (BCIP) and Nitro Blue Tetrazolium (NBT/Thiazolyl Blue/Nitro BT)

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Alkaline Phosphatase Blue Microwell Substrate

sufficient for 400 mL working substrate

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