B1656 Sigma

Monoclonal Anti-Rat Kappa & Lambda Light Chains−Biotin antibody produced in mouse

clone RT-39/RL-6, purified immunoglobulin, buffered aqueous solution

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Synonym: Monoclonal Anti-Rat Kappa & Lambda Light Chains

Properties

Related Categories Alphabetical Index, Antibodies, Biotin Antibodies, Clone Index, Primary Antibodies,
antibody form   purified immunoglobulin
clone   RT-39/RL-6, monoclonal
form   buffered aqueous solution
mol wt   antigen mol wt 25 kDa
application(s)   direct ELISA: 1:40,000
  dot blot: 1:4,000
  immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:600
  western blot (chemiluminescent): 1:40,000
isotype   IgG1/IgG2a
shipped in   dry ice
storage temp.   −20°C

Description

Specificity

The product recognizes epitopes located on the rat κ and λ light chains on the various rat immunoglobulin classes and subclasses. The epitope recognized on the κ light chains is shared by both the 1a and 1b allotypes of this molecule. The antibody detects the κ and λ light chains derived from normal serum immunoglobulins or from myeloma proteins. Weak cross-reaction is observed with guinea pig immunoglobulins but not with human, bovine, cat, chicken, dog, goat, horse, mouse, pig, rabbit or sheep immunoglobulins.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide

General description

The product is a mixture derived from hybridomas RT-39 and RL-6, secreting monoclonal antibodies against rat κ and λ light chains, respectively.

Price and Availability

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