Synonym: BCIP®, X-phosphate disodium salt
|Related Categories||Alkaline Phosphatase, Alphabetical Listing, BCIP/NBT Substrate Systems, Biochemicals and Reagents, Colorimetric,|
|solubility||H2O: soluble20 mg/mL|
1, 5 g in poly bottle
500 mg in poly tube
25, 50, 100 mg in poly bottle
Histochemical substrate for alkaline phosphatase.
For the colorimetric detection of alkaline phosphatase-labeled molecules, 5-Bromo-4-chloro-3-indoly
BCIP is provided in two salt forms: the disodium salt which is soluble in water and the p-toluidine form which is soluble in dimethylformamide. These salt forms may be used to prepare a stock solution that in combination with NBT and a reaction buffer, form a substrate solution for alkaline phosphatase. This substrate system, when incubated with alkaline phosphatase, produces an insoluble NBT diformazan product that is easily observable with its purple color. For added convenience, a mixture of BCIP and NBT is provided in an easily dissolvable tablet form or as a ready-to-use liquid.
BCIP is a registered trademark of Sigma-Aldrich Co. LLC
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Certificate of Analysis
Certificate of Origin
Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrat...
BioFiles 2008, 3.4, 6.
Keywords: Enzyme-linked immunosorbent assay, Immunohistochemistry, Western blot
Rapid and sensitive colorimetric method for visualizing biotin-labeled DNA probes hybridized to DNA or RNA immobilized on nitrocellulose: Bio-blots. Leary, J.J., et al. Proc. Natl. Acad. Sci. U. S. A. 80, 4045-4049, (1983)
Enhanced immunohistochemical detection of autonomic nerve fibers, cytokines and inducible nitric oxide synthase by light and fluorescent microscopy in rat spleen. Meltzer, J.C., et al. J. Histochem. Cytochem. 45(4), 599-610, (1997)
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