5-Bromo-4-chloro-3-indolyl β-D -galactopyranoside
≥98%
DOWNLOAD MSDS (PDF)Synonym:
5-
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CAS Number 7240-90-6
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Empirical Formula (Hill Notation) C14H15BrClNO6
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Molecular Weight 408.63
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Beilstein Registry Number 1402009
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EC Number 230-640-8
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MDL number MFCD00005666
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PubChem Substance ID 24892124
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Popular Documents: Product Information Sheet (PDF) | Datasheet (PDF) | Specification Sheet (PDF)
Properties
| Related Categories | Chromogenic, Galactoside, Gene Reporter Substrates, Glycobiology, Inhibitors and Substrates, |
| assay | ≥98% |
| suitability | suitable for identification of lac+ bacterial colonies |
| storage temp. | −20°C |
Description
Application
X-Gal is a chromogenic substrate for β-galactosidase that produces a rich blue color that can easily be detected visually over background. X-Gal is the substrate of choice for blue-white selection of recombinant bacterial colonies with the lac+ genotype.
Reconstitution
A stock solution (100 mg/ml) is prepared by dissolving X-Gal in dimethylformamide and should be stored at −20 °C and protected from light. Sterilization is not required.
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