|Related Categories||Chromogenic, Galactoside, Gene Reporter Substrates, Glycobiology, Inhibitors and Substrates,|
|suitability||suitable for identification of lac+ bacterial colonies|
X-Gal is a chromogenic substrate for β-galactosidase that produces a rich blue color that can easily be detected visually over background. X-Gal is the substrate of choice for blue-white selection of recombinant bacterial colonies with the lac+ genotype.
A stock solution (100 mg/ml) is prepared by dissolving X-Gal in dimethylformamide and should be stored at −20 °C and protected from light. Sterilization is not required.
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for selection of recombinant bacterial clones
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From our library of Protocols, Sigma-Aldrich presents Microbial Growth Protocols
From our library of Protocols, Sigma-Aldrich presents Transformation Protocols
Keywords: Antibiotics, Detergents, Electrophoresis, Gel electrophoresis, Mass spectrometry, Peptide synthesis, transformation
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Genetic transformation of a clinical (genital tract), plasmid-free isolate of Chlamydia trachomatis: engineering the plasmid as a cloning vector. Wang Y, Kahane S, Cutcliffe LT, et al. PLoS ONE 8(3), e59195, (2013)
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