Popular Documents: Properties
| Related Categories | Bacterial Cell Lysis, Cell Lysis, Cell Lysis and Protein Extraction, Core Bioreagents, Life Science Reagents for Protein Expression and Purification, |
| shipped in | wet ice |
| storage temp. | −20°C |
Description
Application
CelLytic Express is a non-denaturing and highly efficient protein extraction formulation for in-culture bacterial cell lysis. This proprietary powder formulation extracts 2-3 times more protein than conventional methods such as sonication. In addition, the method is fast and requires less sample manipulations, reducing proteolytic degradation and preserving protein activity. Unlike other in-culture lysis products, CelLytic Express is a complete formulation including lysozyme and endonuclease. The resulting lysate is completely clear of cellular debris, and is immediately ready for affinity purification. CelLytic Express′ unique powder formulation adds minimal volume to the final lysate. It provides a cell lysis method with defined volume and time parameters, making it easier to validate and more convenient for production scale protocols.
Features and Benefits
• Efficient and non-denaturing in-culture protein extraction
• Save time by eliminating cell harvest and clarification steps
• Preserve biological activity with fewer manipulations
• Compatible with affinity resins and magnetic beads
• Ideal for Production Scale Extraction protocols
Legal Information
CelLytic is a trademark of Sigma-Aldrich Co. LLC
Price and Availability
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tablet sufficient for 1 mL lysis (cell suspension)
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