Cytochrome P450 Microsome Preparation human
2C8 Isozyme, recombinant, expressed in Saccharomyces cerevisiae, coexpressed with human NADPH reductase and cytochrome b5
DOWNLOAD MSDS (PDF)Synonym: Cerosomes
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Properties
| Related Categories | Application Index, Biochemicals and Reagents, Cell Signaling Enzymes, Cerosomes Cytochrome P450 (CYP) Active Microsome Preparations, Cytochrome P450s (CYPs), |
| recombinant | expressed in Saccharomyces cerevisiae |
| activity | 0.050-0.100 units/pmol enzyme |
| secondary activity | 1700-2300 units/mg protein (Reductase activity) |
| shipped in | dry ice |
| storage temp. | −70°C |
Description
Analysis Note
CYP450 content: 55.0-75.0 picomoles per mg protein
Cyrotchrome b5 content: 200-300 picomoles per mg protein
Other Notes
For more more information visit our drug metabolism resources for Cytochrome P450 microsome preparations.
Unit Definition
P450 Activity: One unit will oxidize 1.0 picomole of dibenzylfluorescein per minute at pH 7.4 at 37 °C.
Reductase Activity: One unit will reduce 1 nanomole of cytochrome c per minute in the presence of NADPH at pH 7.8 at 25 °C.
Physical form
Supplied as a solution containing 50 mM potassium phosphate, pH 7.4, 5% glycerol, 1 mM EDTA, 1 mM PMSF, and 2 mM DTT
Application
Cytochrome P450 Microsome Preparation Human is recombinant and prepared from Saccharomyces cervisiae expressing the human cytochrome 2C8 Isozyme coexpressed with human NADPH reductase and cytochrome b5. It is used to catalyze the oxidative metabolism of xenobiotics and to study the biotransformation and elimination of drugs and pollutants from the body. Cytochrome P450 is used to study hormone synthesis and breakdown, cholesterol synthesis, drug metabolism and bioactivation and vitamin D metabolism1.
Biochem/physiol Actions
Microsomal cytochrome P450 enzymes are found primarily in the endoplasmic reticulum of liver tissue. They catalyze the oxidative metabolism of xenobiotics and are the major enzymes involved in drug metabolism and bioactivation1. The reactions are achieved through a mixed monooxygenase system. The cytochrome P450 enzymes range in molecular mass between 45-60 kDa.
Price and Availability
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Related Papers
1. Cytochrome p450 and chemical toxicology Guengerich FP Chem. Res. Toxicol. 21, 70-83, (2008)
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