CelLytic™ B Cell Lysis Reagent
For bacterial cell lysis, 10× concentrate
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MDL number MFCD02097909
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Popular Documents: Bulletin (PDF) | Specification Sheet (PDF)
Properties
| Related Categories | Bacterial Cell Lysis, Cell Lysis, Cell Lysis and Protein Extraction, Core Bioreagents, Life Science Reagents for Protein Expression and Purification, |
Description
Frequently Asked Questions
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Application
A proprietary, non-denaturing formulation of zwitterionic detergents used for the lysis of bacterial cells and extraction of recombinant proteins.
Features and Benefits
• Higher protein extraction efficiency than traditional methods such as sonication and lysozyme
• Scalable for 1 to 25 grams of bacterial cell paste
• No interference with downstream applications such as affinity chromatography, IP, and Western blotting
• Compatible with protease inhibitors, inhibitor cocktails, chaotropes, salts, chelating agents and reducing agents
Other Notes
10X concentrate of detergent blend contains no buffering components, giving the researcher flexibility to integrate the lysis reagent into their desired system. Also amenable to automation protocols for recombinant protein extraction and purification.
Legal Information
Covered by US Patent No 7,282,475 B2 and are sold for research use only. Commercial use requires addtional licenses.
CelLytic is a trademark of Sigma-Aldrich Co. LLC
Price and Availability
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Technical Service:
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
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