|Related Categories||Bacterial Cell Lysis, Cell Lysis, Cell Lysis and Protein Extraction, Core Bioreagents, Life Science Reagents for Protein Expression and Purification,|
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A proprietary, non-denaturing formulation of zwitterionic detergents used for the lysis of bacterial cells and extraction of recombinant proteins.
• Higher protein extraction efficiency than traditional methods such as sonication and lysozyme
• Scalable for 1 to 25 grams of bacterial cell paste
• No interference with downstream applications such as affinity chromatography, IP, and Western blotting
• Compatible with protease inhibitors, inhibitor cocktails, chaotropes, salts, chelating agents and reducing agents
10X concentrate of detergent blend contains no buffering components, giving the researcher flexibility to integrate the lysis reagent into their desired system. Also amenable to automation protocols for recombinant protein extraction and purification.
Covered by US Patent No 7,282,475 B2 and are sold for research use only. Commercial use requires addtional licenses.
CelLytic is a trademark of Sigma-Aldrich Co. LLC
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≥250 units/μL, ≥90% (SDS-PAGE), recombinant, expressed in E. coli, buffered aqueous glycerol solution
For bacterial cell lysis, standard strength
For bacterial cell lysis, 2× concentrate
For bacterial lysis
Certificate of Analysis
|Precautionary statements||P305 + P351 + P338|
|Personal Protective Equipment||Eyeshields, full-face respirator (US), Gloves, multi-purpose combination respirator cartridge (US), type ABEK (EN14387) respirator filter|
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
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