CS0770 Sigma

NDPase (GDPase, UDPase, ADPase) Assay Kit

sufficient for 300 assays (96-well plates)

Properties

Related Categories Cell Analysis, Cell Biology, Cell Signaling and Neuroscience, Cytoskeleton and Extracellular Matrix, Kits More...
usage   sufficient for 300 assays (96-well plates)
shipped in   dry ice
storage temp.   −20°C

Description

Analysis Note

The kit was tested on rat tissue extracts (liver, kidney, spleen, and heart) and on a yeast (S. cerevisae) extract. It was also tested with microsomal fractions prepared from rat tissues and cell lines.

Application

A simple and reliable method for measuring NDPase activity in sub-cellular fractions based on the detection of the inorganic phosphate released from NDP by the appropriate NDPase. The color formation, measured at 700 nm in the presence of molybdate, is proportional to the amount of the inorganic phosphate released by enzymes.

Unit Definition

1 unit of NDPase releases 1 μmole of inorganic phosphate per minute at 25 °C under conditions specified by the assay.

Biochem/physiol Actions

GDPases/UDPases (NDPases) in yeasts are located exclusively in the Golgi complex and are a convenient marker for Golgi detection. NDPs are degraded to NMPs by nucleoside diphosphatase (GDPase/UDPase). The NMPs are then used in antiport transport of new nucleotide sugars into the organelle lumen. ADPases are known to be expressed on the cell plasma membrane (endothelial cells and vascular precursors) where they participate in nucleotide metabolism.

Price and Availability

Kit component also available separately

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Sodium dodecyl sulfate Solution 10% 5 mL MSDS    
Phosphorus standard Solution 0.65 mM 1.7 mL MSDS    

Kit component only

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ADPase Assay Reagent 10 mL MSDS    
GDPase Assay Reagent 10 mL MSDS    
UDPase Assay Reagent 10 mL MSDS    
Ascorbic Acid Solution 1 mL MSDS    
Molybdenum Color Reagent 60 mL MSDS    

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