CS1050 Sigma

UDP-Galactosyltransferase Assay Kit

sufficient for 100 tests

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Properties

Related Categories Cell Biology, Cell Signaling and Neuroscience, Cytoskeleton and Extracellular Matrix, Kits
usage   sufficient for 100 tests
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... B4GALT1(2683)

Description

Analysis Note

The kit provides a convenient method for quantitative estimation of the UDP-galactosyltransferase activitity in cell or tissue extracts.

Application

The kit assay is based on the transfer of radioactively labeled galactose from UDP-galactose to N-acetyl-D-glucosamine by UDP-galactosyltransferase. The reaction product, a radiolabeled N-acetyl-D-lactosamine, is then purified by ion-exchange chromatography and quantified.

Biochem/physiol Actions

UDP-galactosyltransferase (β4GalT1) is a trans-Golgi membrane bound protein that participates in protein processing in the Golgi apparatus. UDP-galactosyl-transferase catalyzes the transfer of galactose from UDP-galactose to N-acetyl-D-glucosamine. The exclusive location of UDP-galactosyltransferase in the Golgi apparatus in the vast majority of tissues and cells makes it a convenient biochemical marker for Golgi membranes.

Price and Availability

Kit component only

Description

Product #

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Assay Buffer 500 μL    
Manganese Chloride Solution 500 μL    
Acceptor Solution 500 μL    
UDP-Gal Solution 500 μL    
Cleanup Resin 50 mL    
Mini-Columns, capacity 5 mL, overall H 1.5 in. 100 each    
Elution Solution 100 mL    

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