• USA Home

  • D5796 - Dulbecco’s Modified Eagle’s Medium - high glucose

EMAIL THIS PAGE TO A FRIEND

D5796 Sigma

Dulbecco’s Modified Eagle’s Medium - high glucose

With 4500 mg/L glucose, L-glutamine, and sodium bicarbonate, without sodium pyruvate, liquid, sterile-filtered, suitable for cell culture

DOWNLOAD MSDS (PDF)

Synonym: DME, DMEM

Description

Application

Dulbecco′s Modified Eagle′s Medium (DMEM) is a modification of Basal Medium Eagle (BME) that contains four-fold concentrations of the amino acids and vitamins. The original formulation contained 1000 mg/L of glucose and was used to culture embryonic mouse cells. Since then, it has been modified in several ways to support primary cultures of mouse and chicken cells, as well as a variety of normal and transformed cells. Each of these media offers a different combination of L-glutamine and sodium pyruvate. Additionally, the glucose levels have been raised to 4500 mg/L, contributing to the name "DMEM/High".

Linkage

Need FBS? We can assist with your serum planning. Please visit the Serum Planner for more information.

General description

This DMEM-Hi glucose medium is a 1x complete medium with no added factors (common modifications) such as L-alanyl-L-glutamine, HEPES, or sodium pyruvate. It differs from the original DMEM-Hi formulation wherein pyridoxine is substituted for pyridoxal. Pyridoxal is an unstable component of media.

Protocols & Applications

ECACC Handbook-Fundamental Techniques in Cell Culture

Protocol for cyropreservation

Cryostor Cryopreservation Video Protocol

Mycoplasma PCR Detection Kit Video Protocol

Price and Availability

Request a Sample

Serum SamplesShopping around for a sera supplier?
Try Sigma® Life Science!






NEW! Sigma® Cultureware

Cell Culture SuppliesIntroducing Sigma’s new competitively
priced line of cultureware:

  • . Multiwell Plates
  • . Culture Flasks
  • . Cryogenic Vials & More



Customers Also Viewed

D5030Dulbecco′s Modified Eagle′s Medium

Without glucose, L-glutamine, phenol red, sodium pyruvate and sodium bicarbonate, powder, suitable for cell culture

D5671Dulbecco’s Modified Eagle’s Medium - high glucose

With 4500 mg/L glucose and sodium bicarbonate, without L-glutamine and sodium pyruvate, liquid, sterile-filtered, suitable for cell culture, suitable for hybridoma

D6429Dulbecco’s Modified Eagle’s Medium - high glucose

With 4500 mg/L glucose, L-glutamine, sodium pyruvate, and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

D6046Dulbecco’s Modified Eagle’s Medium - low glucose

With 1000 mg/L glucose, L-glutamine, and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

D5546Dulbecco’s Modified Eagle’s Medium - low glucose

With 1000 mg/L glucose, and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?