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D9163 Sigma

DL-Dithiothreitol

for electrophoresis, ≥99%

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Synonym: threo-1,4-Dimercapto-2,3-butanediol, Cleland’s reagent, DTT

DL-Dithiothreitol for electrophoresis, ≥99%
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Description

Analysis Note

Tested for use in reduced SDS-PAGE

Application

An excellent reagent for maintaining SH groups in reduced state; quantitatively reduces disulfides. DTT is effective in sample buffers for reducing protein disulfide bonds prior to SDS-PAGE. DTT can also be used for reducing the disulfide bridge of the cross-linker N,N′-bis(acryloyl)cystamine to break apart the matrix of a polyacrylamide gel. DTT is less pungent and is less toxic than 2-mercaptoethanol. Typically, a seven fold lower concentration of DTT (100 mM) is needed than is used for 2-mercaptoethanol (5% v/v, 700 mM).

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