|Related Categories||DNA Digest Markers, Molecular Biology, Nucleic Acid Electrophoresis, Nucleic Acid Markers|
|grade||for molecular biology|
|usage||100 uses (0.1 mg size)|
|1000 uses (1 mg size)|
|20 uses (20 μg size)|
|500 uses (0.5 mg size)|
|suitability||suitable for electrophoresis (DNA)|
|shipped in||dry ice|
Sigma’s Lambda DNA Hind III Digest is provided in a solution of 10 mM Tris-HCl (pH 8.0), with 1.0 mM EDTA.
Suitable for size determination of dsDNA by electrophoresis with either agarose or polyacrylamide gels.
Sigma’s Lambda DNA Hind III Digest contains 8 fragments from 125 – 23,130 bp. Ten ul of the ladder should be diluted in gel loading buffer and then loaded in a single lane on an agarose or polyacrylamide gel.
Contains 8 fragments, 125–23,130 bp
For optimal resolution, the recommended agarose gel concentration is 0.75%.
Bacteriophage lambda DNA was digested with EcoR I and Hind III to completion, then purified by phenol extraction.
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