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E1270 Sigma

ECM Gel from Engelbreth-Holm-Swarm murine sarcoma

liquid, BioReagent, suitable for cell culture

Synonym: EHS matrix



Related Categories 3D Cell Culture Products, 3D Hydrogels, 3D Scaffolds, 3D Stem Cell Culture Products, Attachment Factors,
Quality Level   PREMIUM
sterility   dialyzed against chloroform
product line   BioReagent
form   liquid
concentration   8 - 12 mg/mL
impurities   endotoxin, tested
suitability   suitable for cell culture
shipped in   dry ice
storage temp.   −20°C
Gene Information   mouse ... Lama1(16772), Lama3(16774), Lama5(16776), Lamb1-1(16777), Lamc1(226519), Lamc2(16782), Nid1(18073), Sdc2(15529)


Frequently Asked Questions

Frequently Asked Questions are available for this Product.


ECM was prepared to a protein concentration of 8-12 mg/mL, containing laminin as a major component, collagen type IV, heparin sulfate proteoglycan, entactin, and other minor components. Addition of collagen type IV to the ECM gel increases polymerization.


ECM gel can used with epithelial cells, endothelial cells, muscle cells, nerve cells and tumor cells.

Biochem/physiol Actions

The ECM gel will undergo thermally activated polymerization when brought to 20-40°C, forming a reconstituted basement membrane. PC12 cells will show neurite formation within 2 days when they are grown on a thin layer of this ECM gel.


For long term storage, keep product at -20°C. The ECM gel may be stored at 2-8°C for up to 72 hours.

Preparation Note

This product is prepared from Engelbreth-Holm-Swarm sarcoma in mice. Gel should be thawed overnight at 2-8°C before use and dispensed to a multiwall plate using a plate and pipettes that are pre-cooled to 2-8°C. The gel may also be diluted up to twofold with 2-8°C Dulbecco′s Modified Eagle′s Medium, and should be done before gel is added to the plate. The product will gel within 5 minutes at 20°C. For prolonged manipulations, work should be conducted below 10°C. Cells can be plated on top of a thin gel layer of 0.5 mm or cultured inside a 1 mm layer. If you culture cells inside, add the cells to the gel prior to plating at a recommended density of 3-4 x 104 cells per mL. To dissociate cells from this gel, use protease in PBS without calcium, magnesium, or EDTA, at a concentration of 0.6-2.4 units per mL.

Price and Availability

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Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 


Certificate of Analysis

Certificate of Origin

Protocols & Articles


Attachment Factors for 3-Dimensional Cell Culture

Three-dimensional (3D) extracellular matrix-based cell culture systems are being used as research tools for understanding normal and disease systems, and for drug screening in vitro. The extracellula...
Efrat Manaster
BioFiles 2008, 3.8, 16.
Keywords: Adhesion, Angiogenesis, Applications, Biomaterials, Cancer, Cell biology, Cell culture, Clinical, Gene expression, Genetic, Genetics, Genomics, Growth factors, Hormones, Molecular biology, Morphogenesis, Protocols, Tools

ECM Gel Cell Attachment Protocol

*In the latter application, cells should be added to the gel prior to plating at a recommended density of 3-4 x 104 cells per mL. To dissociate cells from the gel, use protease (dispase) in PBS witho...
George Sitterley
BioFiles 2008, 3.8, 7.
Keywords: Cell culture, Growth factors, Help

The Cancer Stem Cell Hypothesis

Traditionally, cancer has been viewed as a disease in which environmental or endogenous events induce mutations to critical oncogenes and tumor suppressor genes within a normal cell. The clinical man...
Vicki Caligur
BioFiles 2008, 3.5, 4.
Keywords: Angiogenesis, Apoptosis, Asymmetric synthesis, Cancer, Catalysis, Cell culture, Cell division, Cell proliferation, Clinical, DNA replication, Degradations, Eliminations, Environmental, Events, Gastrointestinal, Gene expression, Growth factors, Ligands, Metabolic Pathways, Phosphorylations, Poisons, Transcription, Transfection, transformation

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