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E1270 Sigma

ECM Gel from Engelbreth-Holm-Swarm murine sarcoma

liquid, BioReagent, suitable for cell culture

Synonym: EHS matrix

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Properties

Related Categories 3D Cell Culture Products, 3D Hydrogels, 3D Scaffolds, 3D Stem Cell Culture Products, Attachment Factors,
Quality Level   PREMIUM
product line   BioReagent
form   liquid
concentration   8 - 12 mg/mL
suitability   suitable for cell culture
shipped in   dry ice
storage temp.   −20°C
Gene Information   mouse ... Lama1(16772), Lama3(16774), Lama5(16776), Lamb1-1(16777), Lamc1(226519), Lamc2(16782), Nid1(18073), Sdc2(15529)

Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Components

ECM was prepared to a protein concentration of 8-12 mg/mL, containing laminin as a major component, collagen type IV, heparin sulfate proteoglycan, entactin, and other minor components. Addition of collagen type IV to the ECM gel increases polymerization.

Application

ECM gel can used with epithelial cells, endothelial cells, muscle cells, nerve cells and tumor cells.

Biochem/physiol Actions

The ECM gel will undergo thermally activated polymerization when brought to 20-40°C, forming a reconstituted basement membrane. PC12 cells will show neurite formation within 2 days when they are grown on a thin layer of this ECM gel.

Caution

For long term storage, keep product at -20°C. The ECM gel may be stored at 2-8°C for up to 72 hours.

Preparation Note

This product is prepared from Engelbreth-Holm-Swarm sarcoma in mice; dialyzed against chloroform. Gel should be thawed overnight at 2-8°C before use and dispensed to a multiwall plate using a plate and pipettes that are pre-cooled to 2-8°C. The gel may also be diluted up to twofold with 2-8°C Dulbecco′s Modified Eagle′s Medium, and should be done before gel is added to the plate. The product will gel within 5 minutes at 20°C. For prolonged manipulations, work should be conducted below 10°C. Cells can be plated on top of a thin gel layer of 0.5 mm or cultured inside a 1 mm layer. If you culture cells inside, add the cells to the gel prior to plating at a recommended density of 3-4 x 104 cells per mL. To dissociate cells from this gel, use protease in PBS without calcium, magnesium, or EDTA, at a concentration of 0.6-2.4 units per mL.

Price and Availability


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Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis. These documents are located on the product detail page under Useful Links & Tools. Click on the following link to search for a Certificate of Analysis. Please click the following link to see the details on our Product Dating Information.
How do I get lot-specific information or a Certificate of Analysis?
A Certificate of Analysis is available by lot number and can be obtained through our Advanced Search Option: http://www.sigmaaldrich.com/catalog/AdvancedSearchPage.do
The data sheet for Product E1270, ECM Gel from Engelbreth-Holm-Swarm murine sarcoma, says that it may be diluted up to twofold. What does that mean?
ECM gel can be diluted twofold with cold Dulbecco's Modified Eagle's Medium (DME).  This means 1 part ECM gel and 1 part DME.
Are ECM Gels, Products E1270 and E6909, the same as Matrigel (from BD)?
Product No. E1270, ECM gel is the equivalent of Matrigel from BD only produced by Sigma-Aldrich.
Are Products E1270 and E6909, ECM Gel from Engelbreth-Holm-Swarm murine sarcoma, growth factor reduced like Matrigel (from BD)?
Product No. E1270 is not growth factor reduced. Product No. E6909 is ECM gel, growth factor reduced.
Do Products E1270 and E6909, ECM Gel from Engelbreth-Holm-Swarm murine sarcoma, contain phenol red?
Product E1270 ECM gel does contain phenol-red from the Dulbecco's modified Eagle's medium component of the product.  Product No. E6909, ECM gel, growth factor reduced, is phenol red free.
What is the calcium concentration in Products E1270, ECM Gel from Engelbreth-Holm-Swarm murine sarcoma?
The approximate concentration of CaCl2 in the ECM solution is 0.265 mg/mL.
How can I retrieve my cells from Product E1270,  ECM Gel from Engelbreth-Holm-Swarm murine sarcoma?
Dispase (Product No. D4818) is a Bacillus-derived neutral protease that is recommended for recovering cells cultured on ECM (Product No. E1270) or Matrigel. Dispase will yield a single cell suspension far more effectively than trypsin, collagenase, or other proteolytic enzymes. Dispase is gentle and will not damage cells harvested for sub-cultivation or other study.
How do I find price and availability?
There are several ways to find pricing and availability for our products.  Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers. 
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS. To access the shipping information for this material, use the link on the product detail page for the product, or search here. 
My question is not addressed here, how can I contact Technical Service for assistance?
Use the option to the right to "Ask a Question" by email of a Technical Service Scientist.
  What kind of solution is the A5955 - Antibiotic Antimycotic Solution (100×), Stabilized product suspended in?
A5955 - Antibiotic Antimycotic Solution (100×), Stabilized is supsended in a sodium citrate buffer solution. Source: Israel production group
Show more questions
Protocols & Articles

Articles

3D Hydrogels

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3D Organoid Culture: New In Vitro Models of Development and Disease

Model systems drive biological research by recapitulating body processes and functions from the molecular to whole organism level. The human body is composed of both cellular and non-cellular materia...
Keywords: Adhesion, Cell attachment, Cell culture, Diseases, Growth factors, Infectious Diseases, Processes and Functions, Vitamins

3D-Cell culture: An overview of advanced tools and techniques

Cell culture techniques are ubiquitous in areas of developmental biology, drug discovery, regenerative medicine and protein production. Since the introduction of cell culture techniques, cells have b...
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Attachment Factors for 3-Dimensional Cell Culture

Three-dimensional (3D) extracellular matrix-based cell culture systems are being used as research tools for understanding normal and disease systems, and for drug screening in vitro. The extracellula...
Efrat Manaster
BioFiles 2008, 3.8, 16.
Keywords: Adhesion, Angiogenesis, Applications, Biomaterials, Cancer, Cell biology, Cell culture, Clinical, Gene expression, Genetic, Genetics, Genomics, Growth factors, Hormones, Molecular biology, Morphogenesis, Protocols, Tools

Cancer Stem Cells: New Targets for Cancer Therapy

Are stem cells involved in cancer? Cancer stem cells (CSC’s) are subpopulations of cancer cells that can self-renew, generate diverse cells in the tumor mass, and sustain tumorigenesis1. Cancer resea...
Keywords: Cancer, Cell attachment, Cell culture, Cell proliferation, Gastrointestinal, Gene expression, Transcription, transformation

ECM hydrogels Cell Attachment Protocol

*To dissociate cells from the gel, use protease (dispase) in PBS without calcium, magnesium, and EDTA, at a concentration of 0.6-2.4 units
Keywords: Antibiotics, Cell culture, Cell disruption, Degradations, Growth factors, Phase transitions

Protocols

3D Neural Stem Cell Models of Alzheimer’s Disease Protocol

Introduction Protocol    • Human ReNcell® VM NSC Maintenance and Passaging    • Viral Infection of Human ReNcell® VM NSCs    • Enrichment of High-Expressing Transgenic Human ReNcell® VM NSCs    • Cul...
Keywords: AGE, Alzheimer Disease, Apoptosis, Cell culture, Cell proliferation, Centrifugation, Deposition, Drug discovery, Flow cytometry, Gene expression, Immunohistochemistry, Microscopy, Optical microscopy, Transcription, Western blot

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Peer-Reviewed Papers
15

References

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