E1270 Sigma

ECM Gel from Engelbreth-Holm-Swarm murine sarcoma

liquid, BioReagent, suitable for cell culture

Synonym: EHS matrix

Properties

Related Categories 3D Matricies for Stem Cell Biology, Attachment Factors, Attachments Factors, Cell Biology, Cell Culture,
Quality Level   PREMIUM
sterility   dialyzed against chloroform
product line   BioReagent
form   liquid
concentration   8 - 12 mg/mL
impurities   endotoxin, tested
suitability   suitable for cell culture
shipped in   dry ice
storage temp.   −20°C
Gene Information   mouse ... Lama1(16772), Lama3(16774), Lama5(16776), Lamb1-1(16777), Lamc1(226519), Lamc2(16782), Nid1(18073), Sdc2(15529)

Description

Frequently Asked Questions

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Application

Epithelial cells, endothelial cells, muscle cells, nerve cells, tumor cells

Recommended for use as a cell culture substratum. For a 24-well plate, use 230-250 μl/well. For a 96-well plate, use 50-100 μl/well. Thaw gel overnight at 2-8 °C before use. The thawed gel may be diluted up to two-fold with cold (2-8 °C) Dulbecco′s Modified Eagle′s Medium. Gel dilutions should be made before it is added to the plate. ECM will gel within 5 minutes at 20 °C. For prolonged manipulations, work should be conducted below 10 °C. Dispense gel to wells of a multiwell plate using pipettes pre-cooled to 2-8 °C. A gel forms at 37 °C and maintains this form with culture medium for at least 14 days. Cells may be plated on top of a thin gel layer (0.5 mm) or cultured inside a 1 mm layer. When cultured inside, cells should be added to the gel prior to plating at a recommended density of 3-4 × 104 cells per mL. To dissociate cells from the gel, use protease (dispase) dissolved in PBS without calcium, magnesium, and EDTA at a working concentration of 0.6-2.4 units/ml.

Caution

ECM gel may be stored up to 72 hours at 2-8 °C.

Other Notes

ECM gel is composed primarily of laminin, collagen type IV, heparan sulfate proteoglycan and entactin. Approximately 8-12 mg/ml basement membrane matrix protein in Dulbecco′s modified Eagle′s medium with 50 μg/ml gentamicin.

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