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Protocols
Cell Quantification
For the majority of manipulations using cell cultures, such as transfections, cell fusion techniques, cryopreservation and subculture routines it is necessary to quantify the number of cells prior to...
Cook Book Sept 2010 Volume 12
Keywords: Carcinogens, Cryopreservation
Cryopreservation of Cell Lines
The protocol below describes the use of passive methods involving an electric -80°C freezer for the cryopreservation of cell cultures. ECACC routinely use a programmable rate controlled freezer. This...
Cook Book Volume 12
Keywords: Cell culture, Cryopreservation, Phase transitions
Resuscitation of Frozen Cell Lines
Many cultures obtained from a culture collection, such as ECACC, will arrive frozen and in order to use the cells they must be thawed and put into culture. It is vital to thaw cells correctly in orde...
Fundamental Techniques in Cell Culture Laboratory Handbook - 2nd Edition
Keywords: Centrifugation, PAGE
Subculture of Adherent Cell Lines
Adherent cell lines will grow in vitro until they have covered the surface area available or the medium is depleted of nutrients. At this point the cell lines should be sub cultured in order to preve...
Cook Book Sept 2010 Volume 12, Fundamental Techniques in Cell Culture Laboratory Handbook-2nd Edition
Keywords: Adhesion
Subculture of Semi-Adherent Cell Lines
Some cultures grow as a mixed population (e.g. B95-8 - marmoset) where a proportion of cells do not attach to the tissue culture fl ask and remain in suspension. Therefore to maintain this heterogene...
Cook Book Sept 2010 Volume 12
Keywords: Cell culture
Subculture of Suspension Cell Lines
In general terms cultures derived from blood (e.g. lymphocytes) grow in suspension. Cells may grow as single cells or in clumps (e.g. EBV transformed lymphoblastoid cell lines). For these types of ce...
Cook Book Sept 2010 Volume 12
Keywords: Centrifugation, Growth factors
Testing for Mycoplasma by Indirect DNA Stain
DNA staining methods such as indirect Hoechst staining techniques are quick with results available within 72 hours which compares favourably with 4 weeks for detection by culture. Staining of culture...
Cook Book Volume 12
Keywords: Cell culture, Detection methods, Evaporation, Indicators, Polymerase chain reaction
Papers
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Identification of genes potentially involved in supporting hematopoietic stem cell activity of stromal cell line MC3T3-G2/PA6. Natsumi Shimizu et al Int. J. Hematol. 87, 239-45, (2008)
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Biomarkers and mechanisms of FANCD2 function. Henning Willers et al J. Biomed. Biotechnol. 2008, 821529, (2008)
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Developmental neurotoxicity of perfluorinated chemicals modeled in vitro. Theodore A. Slotkin et al Environ. Health Perspect. 116, 716-22, (2008)
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Differential protein expression following low temperature culture of suspension CHO-K1 cells. Niraj Kumar et al BMC Biotechnol. 8, 42, (2008)
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CXCL10 blockade protects mice from cyclophosphamide-induced cystitis. Senthilkumar K Sakthivel et al J. Immune Based Ther. Vaccines 6, 6, (2008)
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Receptor tyrosine kinase EphA2 mediates thrombin-induced upregulation of ICAM-1 in endothelial cells in vitro. Barden Chan et al Thromb. Res. Suppl. 123, 745-52, (2009)
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Development of arterial blood supply in experimental liver metastases. Katalin Dezső et al Am. J. Pathol. 175, 835-43, (2009)
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Leukotriene E4-induced pulmonary inflammation is mediated by the P2Y12 receptor. Sailaja Paruchuri et al J. Exp. Med. 206, 2543-55, (2009)
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Modified vaccinia virus Ankara can activate NF-kappaB transcription factors through a double-stranded RNA-activated protein kinase (PKR)-dependent pathway during the early phase of virus replication. Heather E. Lynch et al Virology 391, 177-86, (2009)
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Helicobacter Pylori's plasticity zones are novel transposable elements. Dangeruta Kersulyte et al PLoS ONE 4, e6859, (2009)
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Sodium butyrate activates Notch1 signaling, reduces tumor markers, and induces cell cycle arrest and apoptosis in pheochromocytoma. Max A. Cayo et al Am. J. Transl. Res. 1, 178-83, (2009)
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Selective induction of calcineurin activity and signaling by oligomeric amyloid β. Lindsay C. Reese et al Aging Cell 7, 824-35, (2010)
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Targeted inhibition of interleukin-6 with CNTO 328 sensitizes pre-clinical models of multiple myeloma to dexamethasone-mediated cell death. Peter M. Voorhees et al Br. J. Haematol. 145, 481-90, (2011)
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The glial regenerative response to central nervous system injury is enabled by pros-notch and pros-NFκB feedback. Kentaro Kato et al PLoS Biol. 9, e1001133, (2011)
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Guggulsterone targets smokeless tobacco induced PI3K/Akt pathway in head and neck cancer cells. Muzafar A. Macha et al PLoS ONE 6, e14728, (2011)
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The generation of live offspring from vitrified oocytes. L. Gabriel Sanchez-Partida et al PLoS ONE 6, e21597, (2011)
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A Chinese Herbal Decoction, Danggui Buxue Tang, Stimulates Proliferation, Differentiation and Gene Expression of Cultured Osteosarcoma Cells: Genomic Approach to Reveal Specific Gene Activation Roy C. Y. Choi et al Evid. Based. Complement. Alternat. Med. 2011, DOI: 10.1093/ecam/nen085, (2011)
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Alteration of gene expression by alcohol exposure at early neurulation. Feng C Zhou et al BMC Genomics 12, 124, (2011)
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A eukaryotic-type signalling system of Pseudomonas aeruginosa contributes to oxidative stress resistance, intracellular survival and virulence. Jana Goldová et al BMC Genomics 12, 437, (2011)
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Constitutive, but not challenge-induced, interleukin-10 production is robust in acute pre-pubescent protein and energy deficits: new support for the tolerance hypothesis of malnutrition-associated immune depression based on cytokine production in vivo. Monk JM Int. J. Environ. Res. Public Health 8(1), 117-35, (2011)
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Hydrolyzed eggshell membrane immobilized on phosphorylcholine polymer supplies extracellular matrix environment for human dermal fibroblasts. Ohto-Fujita E Cell Tissue Res. 345(1), 177-90, (2011)
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Cell Uptake and Validation of Novel PECs for Biomedical Applications. Palamà IE J. Drug Deliv. 2011, 203676, (2011)
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Human RNA polymerase II-association factor 1 (hPaf1/PD2) regulates histone methylation and chromatin remodeling in pancreatic cancer. Dey P PLoS ONE 6(10), e26926, (2011)
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The role of calcineurin/NFAT in SFRP2 induced angiogenesis--a rationale for breast cancer treatment with the calcineurin inhibitor tacrolimus. Siamakpour-Reihani S PLoS ONE 6(6), e20412, (2011)
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Evaluation of triblock copolymeric micelles of δ- valerolactone and poly (ethylene glycol) as a competent vector for doxorubicin delivery against cancer. Nair K L J. Nanobiotechnology 9(1), 42, (2011)
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The directionality of the nuclear transport of the influenza A genome is driven by selective exposure of nuclear localization sequences on nucleoprotein. Wu WW Virol. J. 6, 68, (2009)
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2D-PAGE as an effective method of RNA degradome analysis. Nowacka M Mol. Biol. Rep. 39(1), 139-46, (2012)
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Enhancement of allele discrimination by introduction of nucleotide mismatches into siRNA in allele-specific gene silencing by RNAi. Ohnishi Y PLoS ONE 3(5), e2248, (2008)
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Loss of runt-related transcription factor 3 expression leads hepatocellular carcinoma cells to escape apoptosis. Nakanishi Y BMC Cancer 11, 3, (2011)
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Spiny versus stubby: 3D reconstruction of human myenteric (type I) neurons. Lindig TM Histochem. Cell Biol. 131(1), 1-12, (2009)
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