Anti-Mouse IgG (whole molecule)−FITC antibody produced in rabbit

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Immunogen

IgG from pooled normal mouse serum

Pooled normal mouse serum

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Application

Anti-Mouse IgG (whole molecule)-FITC antibody may be used for immunofluorescence at a working antibody dilution of 1:100 on human tonsil sections using mouse monoclonal anti-human IgG as primary antibody. For immunofluorescence of human peripheral blood lymphocytes, a minimum dilution of 1:100 is recommended. For immunohistochemistry applications, the working antibody dilutions range from 1:100 to 1:1000 in various studies. ^1,2,3

Anti-mouse IgG (whole molecule)-FITC antibody (diluted 1:1000) can be used as secondary antibody in immunohistochemistry². It can also be used in immunofluorescence.

General description

IgG antibodies are the smallest immunoglobulins found in all body fluids and can fight against bacterial and viral infections. It is the only type of antibody that can cross the placenta in pregnant woman and give immunity to the foetus⁴. Anti-mouse IgG (whole molecule)-FITC antibody can be used as a secondary antibody in FACS analysis to detect cell surface expression of cd59b and cd59a in cloned CHO cells⁵. Rabbit anti-mouse IgG (whole molecule)-FITC antibody reacts specifically with mouse IgG, IgA and IgM.

IgG antibody subtype is the most abundant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defence of the neonate against infections. Anti-Mouse IgG (whole molecule)-FITC antibody reacts with all mouse IgG subclasses and IgM. Purified rabbit anti-Mouse IgG is conjugated to FITC in an alkaline reaction.