Monoclonal Anti-Grb-2 antibody produced in mouse

Immunogen

synthetic peptide (a.a. 200-217) corresponding to the C-terminal region of human, rat and mouse Grb2.

General description

Grb2 is an adapter protein with one Src homology 2 (SH2) domain and two SH3 domains that facilitate protein-protein interactions. ^2,3 grb2 is highly conserved across species and is critical for development events such as epithelial morphogenesis, cell motility and vasculogenesis. ⁴ Grb2 is closely associated with Sos and activates Ras and the MAPK cascade. Grb2 interacts with signalling proteins involved in T-cell development, B-cell activation and development and autoimmunity. ^5,6 Recent reports implicates Grb2 role in several oncogenic signalling pathways that lead to CML and breast cancer.^7,8
Monoclonal Anti-Grb2 reacts specifically with Grb2 (24 kDa).

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Preparation Note

Purified from culture supernatant of hybridoma cells grown in a bioreactor.

Application

Anti-Grb-2 antibody may be used at a working concentration of 1-2 μg/mL for detection by immunoblotting in whole cell extracts of human T cell leukemia, Jurkat cells. Detection is also possible in extracts of murine spermatozoa by immunoblotting at a working dilution of 1:1000. ¹ The antibody is suitable for indirect ELISA, immunocytochemistry, immunohistochemistry, immunoprecipitation and protein microarray.

Features and Benefits

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