|Related Categories||Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Kinase/Phosphatase Biology, Antibodies in Array,|
|species reactivity||human, mouse, rat|
|immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable|
|indirect ELISA: suitable|
|western blot: 1-2 μg/mL using a whole extract of cultured human acute T-cell leukemia Jurkat cells|
|antibody form||purified immunoglobulin|
|form||buffered aqueous solution|
|mol wt||antigen mol wt 24 kDa|
|shipped in||dry ice|
human ... GRB2(2885)|
mouse ... Grb2(14784)
rat ... Grb2(81504)
synthetic peptide (a.a. 200-217) corresponding to the C-terminal region of human, rat and mouse Grb2.
Grb2 is an adapter protein with one Src homology 2 (SH2) domain and two SH3 domains that facilitate protein-protein interactions. 2,3 grb2 is highly conserved across species and is critical for development events such as epithelial morphogenesis, cell motility and vasculogenesis. 4 Grb2 is closely associated with Sos and activates Ras and the MAPK cascade. Grb2 interacts with signalling proteins involved in T-cell development, B-cell activation and development and autoimmunity. 5,6 Recent reports implicates Grb2 role in several oncogenic signalling pathways that lead to CML and breast cancer.7,8
Monoclonal Anti-Grb2 reacts specifically with Grb2 (24 kDa).
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Purified from culture supernatant of hybridoma cells grown in a bioreactor.
Anti-Grb-2 antibody may be used at a working concentration of 1-2 μg/mL for detection by immunoblotting in whole cell extracts of human T cell leukemia, Jurkat cells. Detection is also possible in extracts of murine spermatozoa by immunoblotting at a working dilution of 1:1000. 1 The antibody is suitable for indirect ELISA, immunocytochemistry, immunohistochemistry, immunoprecipitation and protein microarray.
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Keywords: Buffers, Cell biology, Epigenetics, Immunohistochemistry, Immunoprecipitation, Molecular biology, Neuroscience, Western blot
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