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GC5 chemically competent cells are comparable to the popular DH5α™ strain and carry recA1 and endA1 mutations that aid in plasmid stability and improved quality of prepared plasmid DNA. The GC5 strain allows for beta-galactosidase α-complementation for blue/white screening and is T1 bacteriophage resistant. GC5 Competent Cells are offered as a high efficiency grade for subcloning and generation of cDNA libraries.
The transformation efficiency of GC5 Uni-packs (50 μl single use aliquots) is 1 × 109 cfu/μg pUC19 plasmid DNA. pUC19 Control DNA is provided.
For more information on competent cell selection, the definition of genotypes and to view a presentation on competent cells, please visit www.sigma-aldrich.com/com
Genotype of GC5:
F- F80lacZDM15 D(lacZYA-argF)U169 endA1 recA1relA1 gyrA96 hsdR17 (rk-, mk+) phoA supE44 thi-1 l–T1R
DH5α is a trademark of Invitrogen Corp.
GC5 is a trademark of GeneChoice, Inc.
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