|Related Categories||Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies to Cellular Antigens, Antibodies to Cytoskeleton,|
|application(s)||agglutination assay: 1:50 using human erythrocytes|
|flow cytometry: suitable using bone marrow nucleated cells|
|indirect immunofluorescence: suitable using bone marrow nucleated cells|
|western blot: suitable using extracts of human red blood cell ghosts|
|antibody form||purified immunoglobulin|
|shipped in||dry ice|
|Gene Information||human ... GYPC(2995)|
Solution in 0.1 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.
By immunoblotting, the antibody localizes specifically the Gerbich blood group antigen-related sialoglycoprotein β in extracts of human red blood cell ghosts. The antibody (also cited as clone no. 2C10) binds to 18% of bone marrow nucleated cells in smears and tissue preparations using immunofluorescent microscopy or flow cytometry.
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Keywords: Buffers, Cell biology, Epigenetics, Immunohistochemistry, Immunoprecipitation, Molecular biology, Neuroscience, Western blot
Human erythrocyte antigens. III. Characterization of a panel of murine monoclonal antibodies that react with human erythrocyte and erythroid precursor membranes. Telen, M.J., et al. Vox Sang. 52, 236, (1987)
Construction of recombinant hemagglutinin derived from the gingipain-encoding gene of Porphyromonas gingivalis, identification of its target protein on erythrocytes, and inhibition of hemagglutination by an interdomain regional peptide. Kamaguchi, A., et al. J. Bacteriol. 189, 3977-3986, (2007)
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