Synonym: Antibiotic G418
|Related Categories||A - K, Aminoglycosides, Antibacterial, Antibiotic Solutions, Antibiotics,|
|concentration||50 mg/mL in H2O|
|suitability||suitable for cell culture|
Mode of Action: Blocks polypeptide synthesis and inhibits elongation in the selection and maintenance of eucaryotic cells stably transfected with neomycin resistance gene
50 mg/mL in tissue culture grade water
G 418 is an aminoglycoside antibiotic similar in structure to gentamycin. It is normally toxic towards eukaryotic and prokaryotic cells, but is useful for selection of cells stably transfected with neomycin resistance gene (Neo). The optimal concentration for selection and maintenance must be determined for each cell line. For bacteria and algae, concentrations of 5 μg/ml or less are recommended. Animal cells may require up to 300-500 μg/ml. Typically, resistance is conferred by one of two dominant genes of bacterial origin, which can be expressed in eukaryotic cells. Cells that are multiplying will be affected sooner than those are quiescent. Cells in log phase may require three to seven days for selection. In general, concentrations of approximately 400 μg/ml for selection and 200 μg/ml for maintenance are required for mammalian cells.
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potency: ≥450 μg per mg (dried basis)
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50 mg/mL in deionized water, liquid, sterile-filtered, BioReagent, suitable for cell culture
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