G 418 disulfate salt solution
50 mg/mL in H2O, sterile-filtered, BioReagent, suitable for cell culture
DOWNLOAD MSDS (PDF)Synonym: Antibiotic G418
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CAS Number 108321-42-2
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Empirical Formula (Hill Notation) C20H40N4O10 · 2H2SO4
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Molecular Weight 692.71
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MDL number MFCD00058314
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PubChem Substance ID 24895333
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Popular Documents: Product Information Sheet (PDF)
Properties
| Related Categories | A - K, Aminoglycosides, Antibacterial, Antibiotic Solutions, Antibiotics, |
| sterility | sterile-filtered |
| product line | BioReagent |
| concentration | 50 mg/mL in H2O |
| impurities | endotoxin, tested |
| suitability | suitable for cell culture |
| storage temp. | 2-8°C |
Description
Biochem/physiol Actions
Mode of Action: Blocks polypeptide synthesis and inhibits elongation in the selection and maintenance of eucaryotic cells stably transfected with neomycin resistance gene
Physical form
50 mg/mL in tissue culture grade water
Application
G 418 is an aminoglycoside antibiotic similar in structure to gentamycin. It is normally toxic towards eukaryotic and prokaryotic cells, but is useful for selection of cells stably transfected with neomycin resistance gene (Neo). The optimal concentration for selection and maintenance must be determined for each cell line. For bacteria and algae, concentrations of 5 μg/ml or less are recommended. Animal cells may require up to 300-500 μg/ml. Typically, resistance is conferred by one of two dominant genes of bacterial origin, which can be expressed in eukaryotic cells. Cells that are multiplying will be affected sooner than those are quiescent. Cells in log phase may require three to seven days for selection. In general, concentrations of approximately 400 μg/ml for selection and 200 μg/ml for maintenance are required for mammalian cells.
Protocols & Applications
Price and Availability
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