|Related Categories||Aminoglycosides, Antibiotics, Antibiotics A to Z, Antibiotics G-M, Antibiotics and Antimycotics,|
|purified by||ion-exchange chromatography|
|suitability||plant cell culture tested|
Mode of Action: Blocks polypeptide synthesis and inhibits elongation. For use in the selection and maintenance of prokaryotic and eukaryotic cells.
Hygromycin B is used in molecular biology as a selection agent for plant cells transformed with selectable marker genes such as hygromycin phosphotransferase (hpt).
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plant cell culture tested, BioReagent
powder, BioReagent, suitable for cell culture, suitable for insect cell culture
≥60% (HPAE), 45-60 mg/mL in H2O
≥60% (HPLC), 45-60 mg/mL in H2O, γ-irradiated
Certificate of Analysis
Certificate of Origin
|Symbol||GHS05, GHS06, GHS08|
|Hazard statements||H300 +H310 + H330-H318-H334|
|Precautionary statements||P260-P264-P280-P284-P301 + P310-P302 + P350|
|Hazard Codes (Europe)||T+|
|Risk Statements (Europe)||26/27/28-41-42/43|
|Safety Statements (Europe)||22-26-28-36/37/39-45|
|RIDADR||UN 3462 6.1 / PGI|
Protein synthesis is a complex, multi-step process involving many enzymes as well as conformational alignment. However, the majority of antibiotics that block bacterial protein synthesis interfere wi...
BioFiles 2006, 1.4, 17.
Keywords: Antibiotics, Environmental, Peptide synthesis
1Application Key: CC = Callus Culture; PR = plant regeneration; EC = cotyledon, hypocotyl, or leaf disc culture; TS = transformant selection; SE = somatic embryogenesis; SSC = stem selection culture;...
Keywords: Antibiotics, Cell culture
Development of series of gateway binary vectors, pGWBs, for realizing efficient construction of fusion genes for plant transformation. Nakagawa T, Kurose T, Hino T, Tanaka K, et. al. J. Biosci. Bioeng. 104, 34-41, (2007)
Molecular cloning and characterization of WdTUP1, a gene that encodes a potential transcriptional repressor important for yeast-hyphal transitions in Wangiella (Exophiala) dermatitidis. Liu H Fungal Genet. Biol. 45(5), 646-56, (2008)
Envelope deglycosylation enhances antigenicity of HIV-1 gp41 epitopes for both broad neutralizing antibodies and their unmutated ancestor antibodies. Ma BJ, Alam SM, Go EP, et al. PLoS Pathog. 7(9), e1002200, (2011)
Efficient Agrobacterium-mediated transformation of the liverwort Marchantia polymorpha using regenerating thalli. Kubota A, Ishizaki K, Hosaka M, et al. Biosci. Biotechnol. Biochem. 77(1), 167-72, (2013)
Chiral pool based efficient synthesis of the aminocyclitol core and furanoside of (-)-hygromycin A: formal total synthesis of (-)-hygromycin A. Lo HJ, Chang YK, and Yan TH Org. Lett. 14(23), 5896-9, (2012)
Detection of probable marker-free transgene-positive rice plants resistant to rice tungro disease from backcross progenies of transgenic Pusa Basmati 1. Roy S, Banerjee A, Tarafdar J, et al. J. Genet. 91(2), 213-8, (2012)
Capreomycin and hygromycin B modulate the catalytic activity of the delta ribozyme in a manner that depends on the protonation and complexation with Cu2+ ions of these antibiotics. Szafraniec M, Stokowa-Sołtys K, Nagaj J, et al. Dalton Trans. 41(32), 9728-36, (2012)
Generation of astaxanthin mutants in Xanthophyllomyces dendrorhous using a double recombination method based on hygromycin resistance. Niklitschek M, Baeza M, Fernández-Lobato M, et al. Methods Mol. Biol. 898, 219-34, (2012)
Spontaneous transmitter release recruits postsynaptic mechanisms of long-term and intermediate-term facilitation in Aplysia. Jin I, Udo H, Rayman JB, et al. Proc. Natl. Acad. Sci. U. S. A. 109(23), 9137-42, (2012)
Spontaneous transmitter release is critical for the induction of long-term and intermediate-term facilitation in Aplysia. Jin I, Puthanveettil S, Udo H, et al. Proc. Natl. Acad. Sci. U. S. A. 109(23), 9131-6, (2012)
Accumulation of the FACT complex, as well as histone H3.3, serves as a target marker for somatic hypermutation. Aida M, Hamad N, Stanlie A, et al. Proc. Natl. Acad. Sci. U. S. A. 110(19), 7784-9, (2013)
Generation of Trichoderma atroviride mutants with constitutively activated G protein signaling by using geneticin resistance as selection marker. Gruber S, Omann M, Rodrìguez CE, et al. BMC Res. Notes 5, 641, (2012)
Plant regeneration and genetic transformation of C. canadensis: a non-model plant appropriate for investigation of flower development in Cornus (Cornaceae). Liu X, Feng CM, Franks R, et al. Plant Cell Rep. 32(1), 77-87, (2013)
A system for the measurement of gene targeting efficiency in human cell lines using an antibiotic resistance-GFP fusion gene. Konishi Y, Karnan S, Takahashi M, et al. Biotechniques 53(3), 141-52, (2012)
Agrobacterium tumefaciens-mediated transformation for investigating pathogenicity genes of the phytopathogenic fungus Colletotrichum sansevieriae. Nakamura M, Kuwahara H, Onoyama K, et al. Curr. Microbiol. 65(2), 176-82, (2012)
Expression of wheat Na(+)/H(+) antiporter TNHXS1 and H(+)- pyrophosphatase TVP1 genes in tobacco from a bicistronic transcriptional unit improves salt tolerance. Gouiaa S, Khoudi H, Leidi EO, et al. Plant Mol. Biol. 79(1-2), 137-55, (2012)
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