|Related Categories||8, Alphabetical Index, Antibodies, Antibodies against Cytokines, Chemokines, and Growth Factors for Stem Cell Biology, Antibodies against Cytokines/Growth Factors/Chemokines for Stem Cell Biology,|
|application(s)||flow cytometry: suitable|
|immunohistochemistry: 8-25 μg/mL using human PBMC′s|
|western blot: 1 μg/mL|
|antibody form||purified immunoglobulin|
|Gene Information||human ... IL1B(3553)|
purifed, E. coli-derived, recombinant human IL-1β.
Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline pH 7.4 with 5% trehalose.
The antibody will neutralize the biological activity of recombinant human IL-1β. It will not neutralize the activity of recombinant human IL-α, recombinant mouse IL-1α, or recombinant mouse IL-1β. When immobilized on a microplate, this antibody will capture both recombinant as well as natural human IL-1β. In immunoblotting, the antibody shows ~25% cross-reactivity with recombinant rat IL-1β and <5% cross-reactivity with recombinant mouse IL-1β and recombinant porcine IL-1β. Additionally, in immunoblotting, the antibody exhibits no cross-reactivity with recombinant mouse IL-α, recombinant human IL-1ra, recombinant mouse IL-1ra, or recombinant rat IL-1α.
May used in immunoblotting, immunohistochemistry, ELISA, and neutralization assays
Customers Also Viewed
As a leading supplier of antibodies, Sigma® Life Science is committed to providing researchers the antibody they need with the quality they deserve. Our Antibody Explorer Search Tool provides easy ac...
Keywords: Buffers, Cell biology, Epigenetics, Immunohistochemistry, Immunoprecipitation, Molecular biology, Neuroscience, Western blot
CC and CXC chemokine receptors mediate migration, proliferation, and matrix metalloproteinase production by fibroblast-like synoviocytes from rheumatoid arthritis patients. Alvaro-Gracia, J., et al. Arthritis Rheum. 50, 3866-3877, (2004)
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Need larger quantities for your development, manufacturing or research applications?