Laminin from Engelbreth-Holm-Swarm murine sarcoma basement membrane 1 mg/mL in Tris buffered NaCl, sterile-filtered, BioReagent, suitable for cell culture

Properties

Related Categories	2D Cell Culture, 3D Matricies for Stem Cell Biology, Adhesion, Attachment, and Matrix Factors, Adhesion, Attachment, and Matrix Factors for Stem Cell Expansion, Apoptosis and Cell Cycle, Application Index, Attachment Factors, Attachments Factors, Biochemicals and Reagents, Cell Biology, Cell Culture, Cell Culture Media & Reagents, Cell Signaling Enzymes, Cell Signaling and Neuroscience, Core Bioreagents, Cytoskeleton and Extracellular Matrix, Drug Discovery, ECM Proteins, Enzymes, Inhibitors, and Substrates, Extracellular Matrix, Extracellular Matrix Peptides and Proteins, General Stem Cell Biology, Laminin, Life Science Reagents for Cell Culture, Matrix Metalloproteinases Products, Other Apoptosis Related Reagents, Proteins and Derivatives, Reagents and Supplements, Research Essentials, Stem Cell Biology, Stem Cell Differentiation, Stem Cell Expansion, Structural Proteins More...
Quality Level	PREMIUM
sterility	sterile-filtered
product line	BioReagent
form	aqueous solution
concentration	1 mg/mL in Tris buffered NaCl
surface coverage	1‑2 μg/cm²
suitability	suitable for cell culture
shipped in	dry ice
storage temp.	−20°C
Gene Information	mouse ... Lama1(16772), Lama3(16774), Lama5(16776), Lamb1-1(16777), Lamc1(226519), Lamc2(16782)

Show More (11)

Description

Frequently Asked Questions

Live Chat and Frequently Asked Questions are available for this Product.

Application

Laminin supports growth and differentiation of many cell types including epithelial, endothelial, neural, muscle, and liver cells.

Recommended for use as a cell culture substratum at 1-2 μg/cm². Optimal concentration depends on cell type as well as the application or research objectives.

General description

Laminin, an extracellular matrix (ECM) multidomain trimeric glycoprotein, is the major non-collagenous component of basal lamina that supports adhesion, proliferation and differentiation. It was initially isolated from mouse Engelbreth-Holm-Swarm (EHS) tumor (laminin-1). Laminin proteins are integral components of structural scaffolding in animal tissues. Laminins associate with type IV collagen via entactin and perlecan and bind to cell membranes through integrin receptors, dystrogylcan glycoprotein complex and Lutheran blood group glycoprotein.

Price and Availability

NEW! Sigma^® Cultureware

Introducing Sigma’s new competitively
priced line of cultureware:

. Multiwell Plates
. Culture Flasks
. Cryogenic Vials & More

Learn more

Related Papers

Laminin, proteoglycan, nidogen and collagen IV: structural models and molecular interactions Timpl R, et al. Ciba Found. Symp. 108, 25-43, (1984)

Documents

Certificate of Analysis

Certificate of Origin

Ask A Scientist

L2020 - Product Information Sheet (51 KB)

Safety Information

Personal Protective Equipment	Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter
WGK Germany	3

Technical information & documentation associated with this product is available in the Safety & Documentation tab.

Articles

Attachment Factors for 3-Dimensional Cell Culture

Three-dimensional (3D) extracellular matrix-based cell culture systems are being used as research tools for understanding normal and disease systems, and for drug screening in vitro. The extracellula...
Efrat Manaster
BioFiles 2008, 3.8, 16.
Keywords: Adhesion, Angiogenesis, Biomaterials, Cancer, Cell biology, Cell culture, Clinical, Gene expression, Genetic, Genetics, Genomics, Growth factors, Hormones, Molecular biology, Morphogenesis

Laminin Cell Attachment Protocol

Q1) Can I coat laminin on top of poly-L-lysine for better adherence? A1) When coating coverslips or slides for use with neural stem cells (neurospheres) the following procedure is used. Precoat the c...
George Sitterley
BioFiles 2008, 3.8, 11.
Keywords: PAGE, SDS PAGE

Laminin

Laminin has active domains for collagen binding, cell adhesion, heparin binding, and neurite outgrowth (PHD) fragment.1 Laminin chains are designated A (molecular weight 400kDa), B1 (molecular weight...
George Sitterley
BioFiles 2008, 3.8, 11.
Keywords: Adhesion, Cell culture

The Cancer Stem Cell Hypothesis

Traditionally, cancer has been viewed as a disease in which environmental or endogenous events induce mutations to critical oncogenes and tumor suppressor genes within a normal cell. The clinical man...
Vicki Caligur
BioFiles 2008, 3.5, 4.
Keywords: Angiogenesis, Apoptosis, Asymmetric synthesis, Cancer, Catalysis, Cell culture, Cell division, Cell proliferation, Clinical, DNA replication, Degradations, Eliminations, Environmental, Gastrointestinal, Gene expression, Growth factors, Ligands, Metabolic Pathways, Phosphorylations, Poisons, Transcription, Transfection, transformation