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L2020 Sigma

Laminin from Engelbreth-Holm-Swarm murine sarcoma basement membrane

1-2 mg/mL in Tris buffered NaCl, 0.2 μm filtered, BioReagent, suitable for cell culture

Synonym: Laminin

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Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Components

Laminin is an extracellular matrix multidomain trimeric glycoprotein, and is the main non-collagenous component of basal lamina that supports adhesion, proliferation and differentiation. Laminin is composed of both A, B1 and B2 chains, which are connected by many disulfide bonds. This laminin product was isolated from mouse Engelbreth-Holm-Swarm tumor.

Application

Laminin supports growth and differentiation of many cell types including epithelial, endothelial, neural, muscle and liver cells. It is recommended for use as a cell culture substratum at 1-2 μg/cm2. The optimal concentration does depend on cell type as well as the application or research objectives.

Laminin from Engelbreth-Holm-Swarm murine sarcoma basement membrane has been used in:
• insulin-producing cell (IPC) differentiation.
• Infection inhibition assays.
• Proliferation Assay.

Biochem/physiol Actions

Laminin proteins are integral components of structural scaffolding in animal tissues. They associate with type IV collagen via entactin and perlecan and bind to cell membranes through integrin receptors, dystroglycan glycoprotein complexes and Lutheran blood group glycoproteins. Laminin has active domains for collagen binding, cell adhesion, heparin binding, and neurite outgrowth fragment.

Caution

It is recommended to store this product in working aliquots at -20°C.

Preparation Note

This product is supplied at a concentration of 1 mg/mL in 50 mM Tris-HCl, with 150 mM NaCl. It is filtered using a 0.2μm filter. Thaw this solution slowly before use at 2-8°C to avoid gel formation. For use as a coating, dilute in a balanced salt solution, coat culture surface with a minimal volume and incubate at 37°C for two hours. Wash 3 times with PBS before plating cells. Laminin coatings can be stored for one month at 2-8°C.

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Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis. These documents are located on the product detail page under Useful Links & Tools. Click on the following link to search for a Certificate of Analysis. Please click the following link to see the details on our Product Dating Information.
What is the protein concentration of the solution of Product L2020, Laminin?
The protein specification for L2020 is 1.0 to 1.3 mg/mL. The exact value can be found on the lot-specific Certificate of Analysis, which can be downloaded from our website. Here is the link to the product detail page for L2020: http://www.sigmaaldrich.com/catalog/ProductDetail.do?lang=en&N4=L2020|SIGMA&N5=SEARCH_CONCAT_PNO|BRAND_KEY&F=SPEC Enter the lot numer in the box in the section of the product page called Useful Links & Tools.
What type of laminin is Product L2020?
In the nomenclature system by Burgeson, this is Laminin 1. The laminins were numbered with Arabic numerals in the order discovered. The previous A, B1, and B2 chains, and their isoforms are listed by the Greek letters, for alpha, beta, and gamma, respectively, followed by an Arabic numeral to identify the isoforms.  For example, the first laminin identified from the Engelbreth-Holm-Swarm tumor (EHS) was designated laminin-1 with the chain composition α-1/β-1/γ-1. The genes for these 3 chains are LAMA1, LAMB1, and LAMC1. Reference: Burgeson, R.E., et al., A new nomenclature for the laminins. Matrix Biol., 14, 209-211 (1994).
What is the purity of Laminin, Product L2020?
We do not determine a purity of this product.  An SDS PAGE gel is run and the pattern (3 bands) is comparable to past lots.
Can I coat Laminin, Product L2020, on top of poly-L-lysine for better adherence?
When coating coverslips or slides for use with neural stem cells (neurospheres), the following procedure is recommended. Precoat the coverslips with poly-L-lysine (Product No. P5899) at 100 µg/mL. This is incubated for 30 minutes or more at room temperature. The coverslips are then washed with sterile water and allowed to dry. The poly-lysine coated coverslips can be stored at room temperature until ready to use (up to ~1 month). Coverslips are then coated with 30 - 50 µL of warmed laminin, which is at a concentration of 50 µg/mL. Incubate at 37°C for 30 minutes. Remove the laminin, and wash with DME (50 µL) twice. Cells are then added to the coverslips.
How can I store solutions of Laminin, Product L2020?
The product has a recommended storage temperature of -20°C.  Please note, the product needs to be thawed slowly at 2-8°C.  If the product has been warmed up at room temperature and it gels, it cannot be reactivated for use. We recommend storage at 2-8°C for up to one week. For longer storage, the product needs to be frozen at -20°C in single-use aliquots.
How do I get lot-specific information or a Certificate of Analysis?
A Certificate of Analysis is available by lot number and can be obtained through our Advanced Search Option: http://www.sigmaaldrich.com/catalog/AdvancedSearchPage.do
How do I find price and availability?
There are several ways to find pricing and availability for our products.  Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers. 
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS. To access the shipping information for this material, use the link on the product detail page for the product, or search here. 
My question is not addressed here, how can I contact Technical Service for assistance?
Use the option to the right to "Ask a Question" by email of a Technical Service Scientist.
  What kind of solution is the A5955 - Antibiotic Antimycotic Solution (100×), Stabilized product suspended in?
A5955 - Antibiotic Antimycotic Solution (100×), Stabilized is supsended in a sodium citrate buffer solution. Source: Israel production group
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