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L2020 Sigma

Laminin from Engelbreth-Holm-Swarm murine sarcoma basement membrane

1-2 mg/mL in Tris buffered NaCl, sterile-filtered, BioReagent, suitable for cell culture

Synonym: Laminin



Frequently Asked Questions

Live Chat and Frequently Asked Questions are available for this Product.


Laminin is an extracellular matrix multidomain trimeric glycoprotein, and is the main non-collagenous component of basal lamina that supports adhesion, proliferation and differentiation. Laminin is composed of both A, B1 and B2 chains, which are connected by many disulfide bonds. This laminin product was isolated from mouse Engelbreth-Holm-Swarm tumor.


Laminin supports growth and differentiation of many cell types including epithelial, endothelial, neural, muscle and liver cells. It is recommended for use as a cell culture substratum at 1-2 μg/cm2. The optimal concentration does depend on cell type as well as the application or research objectives.

Biochem/physiol Actions

Laminin proteins are integral components of structural scaffolding in animal tissues. They associate with type IV collagen via entactin and perlecan and bind to cell membranes through integrin receptors, dystroglycan glycoprotein complexes and Lutheran blood group glycoproteins. Laminin has active domains for collagen binding, cell adhesion, heparin binding, and neurite outgrowth fragment.


It is recommended to store this product in working aliquots at -20°C.

Preparation Note

This product is supplied at a concentration of 1 mg/mL in 50 mM Tris-HCl, with 150 mM NaCl. It is sterile-filtered using a 0.2μm filter. Thaw this solution slowly before use at 2-8°C to avoid gel formation. For use as a coating, dilute in a sterile balanced salt solution, coat culture surface with a minimal volume and incubate at 37°C for two hours. Wash 3 times with PBS before plating cells. Laminin coatings can be stored for one month at 2-8°C.

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Safety & Documentation

Safety Information

WGK Germany 
Protocols & Articles


Attachment Factors for 3-Dimensional Cell Culture

Three-dimensional (3D) extracellular matrix-based cell culture systems are being used as research tools for understanding normal and disease systems, and for drug screening in vitro. The extracellula...
Efrat Manaster
BioFiles 2008, 3.8, 16.
Keywords: Adhesion, Angiogenesis, Applications, Biomaterials, Cancer, Cell biology, Cell culture, Clinical, Gene expression, Genetic, Genetics, Genomics, Growth factors, Hormones, Molecular biology, Morphogenesis, Protocols, Tools

Laminin Cell Attachment Protocol

Q1) Can I coat laminin on top of poly-L-lysine for better adherence? A1) When coating coverslips or slides for use with neural stem cells (neurospheres) the following procedure is used. Precoat the c...
George Sitterley
BioFiles 2008, 3.8, 11.
Keywords: Biofiles, PAGE, SDS PAGE


Laminin has active domains for collagen binding, cell adhesion, heparin binding, and neurite outgrowth (PHD) fragment.1 Laminin chains are designated A (molecular weight 400kDa), B1 (molecular weight...
George Sitterley
BioFiles 2008, 3.8, 11.
Keywords: Adhesion, Biofiles, Cell culture

The Cancer Stem Cell Hypothesis

Traditionally, cancer has been viewed as a disease in which environmental or endogenous events induce mutations to critical oncogenes and tumor suppressor genes within a normal cell. The clinical man...
Vicki Caligur
BioFiles 2008, 3.5, 4.
Keywords: Angiogenesis, Apoptosis, Asymmetric synthesis, Cancer, Catalysis, Cell culture, Cell division, Cell proliferation, Clinical, DNA replication, Degradations, Eliminations, Environmental, Events, Gastrointestinal, Gene expression, Growth factors, Ligands, Metabolic Pathways, Phosphorylations, Poisons, Transcription, Transfection, transformation

Peer-Reviewed Papers


Set your institution to view full text papers.

Increased BMP6 levels in the brains of Alzheimer's disease patients and APP transgenic mice are accompanied by impaired neurogenesis. Crews L, Adame A, Patrick C, et al. J. Neurosci. 30(37), 12252-62, (2010)


Matrigel: basement membrane matrix with biological activity Kleinman HK, and Martin GR. Semin. Cancer Biol. 15, 378-386, (2005)


Molecular architecture of basement membranes Yurchenco PD, and Schittny JC. FASEB J. 4, 1577-1590, (1990)


Cell-cell and cell-extracellular matrix interactions regulate embryonic stem cell differentiation Chen SS, et al. Stem Cells 25, 553-561, (2007)


Laminin, proteoglycan, nidogen and collagen IV: structural models and molecular interactions Timpl R, et al. Ciba Found. Symp. 108, 25-43, (1984)


Human Embryonic Stem Cell-derived Neural Precursor Transplants In Collagen Scaffolds Promote Recovery In Injured Rat Spinal Cord. Hatami, M., et al. Cytotherapy 11, 618-30, (2009)


Role of laminin carbohydrates on cellular interactions Tanzer ML, et al. Kidney Int. 43, 66-72, (1993)


Establishment of a cell line from the EHS tumor: biosynthesis of basement membrane constituents and characterization of a hybrid proteoglycan containing heparan and chondroitin sulfate chains Danielson KG, et al. Matrix 2, 22-35, (1992)


Upward synaptic scaling is dependent on neurotransmission rather than spiking. Fong MF, Newman JP, Potter SM, et al. Nat. Commun. 6, 6339, (2015)


Duration of culture and sonic hedgehog signaling differentially specify PV versus SST cortical interneuron fates from embryonic stem cells. Tyson JA, Goldberg EM, Maroof AM, et al. Development 142(7), 1267-78, (2015)


GPR56 regulates pial basement membrane integrity and cortical lamination. Shihong Li et al J. Neurosci. 28, 5817-26, (2008)


Developmentally regulated collagen/integrin interactions confer adhesive properties to early postnatal neural stem cells. Bergström T, Holmqvist K, Tararuk T, et al. Biochim. Biophys. Acta 1840(8), 2526-32, (2014)


Generation of rat-induced pluripotent stem cells from a new model of metabolic syndrome. Takenaka-Ninagawa N, Kawabata Y, Watanabe S, et al. PLoS ONE 9(8), e104462, (2014)


Transcripts involved in calcium signaling and telencephalic neuronal fate are altered in induced pluripotent stem cells from bipolar disorder patients. Chen HM, DeLong CJ, Bame M, et al. Transl. Psychiatry 4, e375, (2014)


Development of a pluripotent stem cell derived neuronal model to identify chemically induced pathway perturbations in relation to neurotoxicity: effects of CREB pathway inhibition. Pistollato F, Louisse J, Scelfo B, et al. Toxicol. Appl. Pharmacol. 280(2), 378-88, (2014)


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