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L2020 Sigma

Laminin from Engelbreth-Holm-Swarm murine sarcoma basement membrane

1-2 mg/mL in Tris buffered NaCl, sterile-filtered, BioReagent, suitable for cell culture

Synonym: Laminin

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Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Components

Laminin is an extracellular matrix multidomain trimeric glycoprotein, and is the main non-collagenous component of basal lamina that supports adhesion, proliferation and differentiation. Laminin is composed of both A, B1 and B2 chains, which are connected by many disulfide bonds. This laminin product was isolated from mouse Engelbreth-Holm-Swarm tumor.

Application

Laminin supports growth and differentiation of many cell types including epithelial, endothelial, neural, muscle and liver cells. It is recommended for use as a cell culture substratum at 1-2 μg/cm2. The optimal concentration does depend on cell type as well as the application or research objectives.

Biochem/physiol Actions

Laminin proteins are integral components of structural scaffolding in animal tissues. They associate with type IV collagen via entactin and perlecan and bind to cell membranes through integrin receptors, dystroglycan glycoprotein complexes and Lutheran blood group glycoproteins. Laminin has active domains for collagen binding, cell adhesion, heparin binding, and neurite outgrowth fragment.

Caution

It is recommended to store this product in working aliquots at -20°C.

Preparation Note

This product is supplied at a concentration of 1 mg/mL in 50 mM Tris-HCl, with 150 mM NaCl. It is sterile-filtered using a 0.2μm filter. Thaw this solution slowly before use at 2-8°C to avoid gel formation. For use as a coating, dilute in a sterile balanced salt solution, coat culture surface with a minimal volume and incubate at 37°C for two hours. Wash 3 times with PBS before plating cells. Laminin coatings can be stored for one month at 2-8°C.

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Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles

Articles

Attachment Factors for 3-Dimensional Cell Culture

Three-dimensional (3D) extracellular matrix-based cell culture systems are being used as research tools for understanding normal and disease systems, and for drug screening in vitro. The extracellula...
Efrat Manaster
BioFiles 2008, 3.8, 16.
Keywords: Adhesion, Angiogenesis, Applications, Biomaterials, Cancer, Cell biology, Cell culture, Clinical, Gene expression, Genetic, Genetics, Genomics, Growth factors, Hormones, Molecular biology, Morphogenesis, Protocols, Tools

Laminin Cell Attachment Protocol

Q1) Can I coat laminin on top of poly-L-lysine for better adherence? A1) When coating coverslips or slides for use with neural stem cells (neurospheres) the following procedure is used. Precoat the c...
George Sitterley
BioFiles 2008, 3.8, 11.
Keywords: Biofiles, PAGE, SDS PAGE

Laminin

Laminin has active domains for collagen binding, cell adhesion, heparin binding, and neurite outgrowth (PHD) fragment.1 Laminin chains are designated A (molecular weight 400kDa), B1 (molecular weight...
George Sitterley
BioFiles 2008, 3.8, 11.
Keywords: Adhesion, Biofiles, Cell culture

The Cancer Stem Cell Hypothesis

Traditionally, cancer has been viewed as a disease in which environmental or endogenous events induce mutations to critical oncogenes and tumor suppressor genes within a normal cell. The clinical man...
Keywords: Apoptosis, Asymmetric synthesis, Cancer, Catalysis, Cell division, Cell proliferation, Cell signaling, Clinical, Degradations, Environmental, Gastrointestinal, Gene expression, Ligands, Phosphorylations, Poisons, Transcription, Transfection, transformation

Peer-Reviewed Papers
15

References

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