Lectin from Bandeiraea simplicifolia (Griffonia simplicifolia) Isolectin B4 (BSI-B4), lyophilized powder

Properties

Related Categories	Bandeiraea simplicifolia (Griffonia simplicifolia), Bioactive Small Molecules, Cell Biology, Cell Signaling and Neuroscience, Glycan Recognizing Proteins, Glycobiology, Immune Cell Signaling and Blood, Immune System Regulation, L, Lectins, Lectins and Lectin Antibodies, Post-Translational Modification, Proteomics More...
form	lyophilized powder
potency	≤8 μg per mL agglutination activity (using human blood group B erythrocytes)
	≥125 μg per mL agglutination activity (using human blood group A erythrocytes)
composition	Protein, ~95% E1%/280
storage temp.	−20°C

Description

Analysis Note

Agglutination activity is expressed in μg/mL and is determined from serial dilutions of a 1 mg/mL solution using phosphate buffered saline, pH 6.8, containing, for each lectin, calcium, magnesium, and manganese at different concentrations. This activity is the lowest concentration to agglutinate a 2% suspension of appropriate erythrocytes after 1 hr incubation at 25 °C.

Biochem/physiol Actions

BS-I has a major affinity for terminal α-D-galactosyl residues with a secondary affinity for terminal N-acetyl-α-D-galactosaminyl residues.¹

Other Notes

BS-I is a tetrameric lectin consisting of two types of subunits designated A and B. There are five BS-I isolectins with different subunit composition: BSI-B₄, BSI-AB₃, BSI-A₂B₂, BSI-A₃B and BSI-A₄. BSI-B₄ is blood group B specific and has an exclusive affinity for terminal α-D-galactosyl residues, whereas BSI-A₄ has blood group A specificity and has a major affinity for terminal N-acetyl-α-D-galactosaminyl residues.