L5380 Sigma

Lectin from Pisum sativum (pea)

lyophilized powder

DOWNLOAD MSDS (PDF)

Synonym: Pisum sativum agglutinin, PSA

Properties

Related Categories Pisum sativum (Pea), Bioactive Small Molecules, Cell Biology, Glycan Recognizing Proteins, Glycobiology,
form   lyophilized powder
potency   <20 μg/mL per mL agglutination activity
composition   Protein, ≥70% biuret
impurities   salt, free
storage temp.   2-8°C

Description

Analysis Note

Agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, of a 1 mg/ml solution. This activity is the lowest concentration to agglutinate a 2% suspension of human erythrocytes after 1 hr incubation at 25 °C.

Biochem/physiol Actions

PSA is not blood group specific, but has an affinity for terminal α-D-mannosyl residues. PSA is a mitogen similar to concanavalin A.

Preparation Note

Conjugates are prepared from affinity purified lectin.

Purified by affinity chromatography.

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