LuminoCt^® SYBR^® Green qPCR ReadyMix^™

Application

LuminoCt SYBR Green qPCR ReadyMix is designed to deliver unsurpassed assay speeds without sacrificing accuracy, precision, or sensitivity. Extensive design and validation of the ReadyMix chemistry has virtually eliminated the need for optimization when LuminoCt is used in conjunction with properly designed primers. Inclusion of JumpStart^™ Taq antibody in the ReadyMix eliminates polymerase activity at ambient temperatures without causing the performance issues associated with chemically modified hot-start Taq. This allows for very rapid activation of the Taq and delivers unparalleled assay sensitivity, while allowing for benchtop reaction setup.

• Kit is designed to perform SYBR Green-based qPCR assays on commonly available real-time instrument platforms
• ReadyMix requires the addition of reference dye (provided in kit) when being used on real-time instruments that require ROX passive reference dye for normalization of qPCR assays
• Kit is not compatible with qPCR instruments that utilize glass capillary tubes

Features and Benefits

• Assay results in as little as 25 minutes
• Unsurpassed accuracy, precision, and sensitivity
• Virtually eliminates the need to optimize assay parameters

Other Notes

• At a minimum, use software to design primers for assays and ensure that qPCR amplicons are <200 bp
• Sigma recommends designing primers and probes with Beacon Designer software
• To order primers and probes from Sigma, visit: www.sigma.com/oligos

Packaging

Default reaction volume is 50 μL

100RXN is packaged as 1 X 2.5 mL
500RXN is packaged as 1 X 12.5 mL
2000RXN is packaged as 1 X 50 mL

Legal Information

JumpStart is a trademark of Sigma-Aldrich Co. LLC

LuminoCt is a registered trademark of Sigma-Aldrich Co. LLC

ReadyMix is a trademark of Sigma-Aldrich Co. LLC

SYBR is a registered trademark of Life Technologies