Lectin from Ulex europaeus (gorse, furze) biotin conjugate, lyophilized powder

Properties

Related Categories	Ulex europaeus (Gorse, furze), Bioactive Small Molecules, Cell Biology, Cell Signaling and Neuroscience, Glycan Recognizing Proteins, Glycobiology, Immune Cell Signaling and Blood, Immune System Regulation, L, Lectins, Lectins and Lectin Antibodies, Post-Translational Modification, Proteomics More...
form	lyophilized powder
potency	<50 μg/mL agglutination activity
composition	Protein, ~85% E1%/280
extent of labeling	~3 mol biotin per mol protein
conjugate	biotin conjugate
storage temp.	2-8°C

Description

Analysis Note

Agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, of a 1 mg/ml solution. This activity is the lowest concentration to agglutinate a 2% suspension of human blood group O erythrocytes after 1 hour incubation at 25 °C.

Biochem/physiol Actions

Ulex europaeus agglutinin has anti-H blood group specificity. There are two types of lectin: UEA I has an affinity for L-fucose and UEA II has an affinity for N,N′-diacetylchitobiose. The mol. wt. of UEA I was initially found to be 170,000 daltons. Later reports indicate that UEA I may form aggregates at neutral and basic pH and that the correct mol. wt. is 68,000 daltons.