Methionine Sulfoxide Reductase A, histidine-tagged human recombinant, expressed in E. coli, ≥8 units/mg protein, buffered aqueous solution

Properties

Related Categories	Cell Biology, Cell Signaling and Neuroscience, Cell Stress, Enzymes, Nitric Oxide and Cell Stress, Oxidative Stress Enzymes More...
recombinant	expressed in E. coli
assay	≥90% (SDS-PAGE)
form	buffered aqueous solution
shipped in	wet ice
storage temp.	2-8°C
Gene Information	human ... MSRA(4482)

Description

Analysis Note

The assay is based on the ability of MsrA to reduce methionine sulfoxide, which is measured by monitoring the level of NADPH oxidation in the presence of a thioredoxin-regenerating system.

Unit Definition

One unit will cause the oxidation of 1 μmole of NADPH per minute at 37 °C at pH 7.6.

Physical form

Supplied in a solution of 50 mM Tris, pH 7.6, and 150 mM NaCl.

Biochem/physiol Actions

Methionine (Met) residues of proteins are readily oxidized to methionine sulfoxide (MetO), especially under oxidative stress conditions. Oxidative alteration of Met to R/S-Met(O) sterioisomers is reversed by methionine sulfoxide reductases: MsrA reduces S-MetO and MsrB reduces R-MetO, which may prevent irreversible oxidative protein damage and thus extend an organism′s life span.^1,² A series of proteins have been identified as substrates of MsrA, these include Calmodulin and HIV protease. There is evidence showing a connection between MsrA and Alzheimer′s disease.³